Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367050846> ?p ?o ?g. }
- W4367050846 abstract "Abstract Background The continuous proliferation of intestinal stem cells followed by their tightly regulated differentiation to epithelial cells is essential for the maintenance of the gut epithelial barrier and its functions. How these processes are tuned by diet and gut microbiome is an important, but poorly understood question. Dietary soluble fibers, such as inulin, are known for their ability to impact the gut bacterial community and gut epithelium, and their consumption has been usually associated with health improvement in mice and humans. In this study, we tested the hypothesis that inulin consumption modifies the composition of colonic bacteria and this impacts intestinal stem cells functions, thus affecting the epithelial structure. Methods Mice were fed with a diet containing 5% of the insoluble fiber cellulose or the same diet enriched with an additional 10% of inulin. Using a combination of histochemistry, host cell transcriptomics, 16S microbiome analysis, germ-free, gnotobiotic, and genetically modified mouse models, we analyzed the impact of inulin intake on the colonic epithelium, intestinal bacteria, and the local immune compartment. Results We show that the consumption of inulin diet alters the colon epithelium by increasing the proliferation of intestinal stem cells, leading to deeper crypts and longer colons. This effect was dependent on the inulin-altered gut microbiota, as no modulations were observed in animals deprived of microbiota, nor in mice fed cellulose-enriched diets. We also describe the pivotal role of γδ T lymphocytes and IL-22 in this microenvironment, as the inulin diet failed to induce epithelium remodeling in mice lacking this T cell population or cytokine, highlighting their importance in the diet-microbiota-epithelium-immune system crosstalk. Conclusion This study indicates that the intake of inulin affects the activity of intestinal stem cells and drives a homeostatic remodeling of the colon epithelium, an effect that requires the gut microbiota, γδ T cells, and the presence of IL-22. Our study indicates complex cross kingdom and cross cell type interactions involved in the adaptation of the colon epithelium to the luminal environment in steady state." @default.
- W4367050846 created "2023-04-27" @default.
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- W4367050846 date "2023-04-26" @default.
- W4367050846 modified "2023-10-18" @default.
- W4367050846 title "Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium" @default.
- W4367050846 cites W1483496013 @default.
- W4367050846 cites W1831301545 @default.
- W4367050846 cites W1908157110 @default.
- W4367050846 cites W1968797543 @default.
- W4367050846 cites W1980801985 @default.
- W4367050846 cites W1981177186 @default.
- W4367050846 cites W1982603620 @default.
- W4367050846 cites W2006522099 @default.
- W4367050846 cites W2008285918 @default.
- W4367050846 cites W2037347206 @default.
- W4367050846 cites W2048469790 @default.
- W4367050846 cites W2048604446 @default.
- W4367050846 cites W2059364985 @default.
- W4367050846 cites W2074337844 @default.
- W4367050846 cites W2081904793 @default.
- W4367050846 cites W2102121200 @default.
- W4367050846 cites W2108865848 @default.
- W4367050846 cites W2109963121 @default.
- W4367050846 cites W2112162638 @default.
- W4367050846 cites W2113292303 @default.
- W4367050846 cites W2115800864 @default.
- W4367050846 cites W2119333069 @default.
- W4367050846 cites W2121836743 @default.
- W4367050846 cites W2133856765 @default.
- W4367050846 cites W2138411980 @default.
- W4367050846 cites W2141304385 @default.
- W4367050846 cites W2145955680 @default.
- W4367050846 cites W2149515881 @default.
- W4367050846 cites W2152885278 @default.
- W4367050846 cites W2159861680 @default.
- W4367050846 cites W2165067288 @default.
- W4367050846 cites W2179438025 @default.
- W4367050846 cites W2190545194 @default.
- W4367050846 cites W2195777839 @default.
- W4367050846 cites W2243046831 @default.
- W4367050846 cites W2268221626 @default.
- W4367050846 cites W2292760617 @default.
- W4367050846 cites W2332292689 @default.
- W4367050846 cites W2335805754 @default.
- W4367050846 cites W2401404581 @default.
- W4367050846 cites W2409656339 @default.
- W4367050846 cites W2416508320 @default.
- W4367050846 cites W2439651008 @default.
- W4367050846 cites W2463118776 @default.
- W4367050846 cites W2519079896 @default.
- W4367050846 cites W2520176593 @default.
- W4367050846 cites W2552041992 @default.
- W4367050846 cites W2573436773 @default.
- W4367050846 cites W2617316973 @default.
- W4367050846 cites W2621499822 @default.
- W4367050846 cites W2627092521 @default.
- W4367050846 cites W2734790731 @default.
- W4367050846 cites W2735797201 @default.
- W4367050846 cites W2743502656 @default.
- W4367050846 cites W2754761629 @default.
- W4367050846 cites W2776874704 @default.
- W4367050846 cites W2780398151 @default.
- W4367050846 cites W2782187941 @default.
- W4367050846 cites W2791416031 @default.
- W4367050846 cites W2803398082 @default.
- W4367050846 cites W2808386728 @default.
- W4367050846 cites W2811149247 @default.
- W4367050846 cites W2814562104 @default.
- W4367050846 cites W2886401819 @default.
- W4367050846 cites W2890605864 @default.
- W4367050846 cites W2895456557 @default.
- W4367050846 cites W2898068898 @default.
- W4367050846 cites W2901254075 @default.
- W4367050846 cites W2912761389 @default.
- W4367050846 cites W2916244827 @default.
- W4367050846 cites W2917960454 @default.
- W4367050846 cites W2920984735 @default.