Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367054436> ?p ?o ?g. }
- W4367054436 endingPage "3711" @default.
- W4367054436 startingPage "3711" @default.
- W4367054436 abstract "A typical bottom-up proteomic workflow comprises sample digestion with trypsin, separation of the hydrolysate using reversed-phase HPLC, and detection of peptides via electrospray ionization (ESI) tandem mass spectrometry. Despite the advantages and wide usage of protein identification and quantification, the procedure has limitations. Some domains or parts of the proteins may remain inadequately described due to inefficient detection of certain peptides. This study presents an alternative approach based on sample acetylation and mass spectrometry with atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). These ionizations allowed for improved detection of acetylated peptides obtained via chymotrypsin or glutamyl peptidase I (Glu-C) digestion. APCI and APPI spectra of acetylated peptides often provided sequence information already at the full scan level, while fragmentation spectra of protonated molecules and sodium adducts were easy to interpret. As demonstrated for bovine serum albumin, acetylation improved proteomic analysis. Compared to ESI, gas-phase ionizations APCI and APPI made it possible to detect more peptides and provide better sequence coverages in most cases. Importantly, APCI and APPI detected many peptides which passed unnoticed in the ESI source. Therefore, analytical methods based on chymotrypsin or Glu-C digestion, acetylation, and APPI or APCI provide data complementary to classical bottom-up proteomics." @default.
- W4367054436 created "2023-04-27" @default.
- W4367054436 creator A5003313570 @default.
- W4367054436 creator A5009480174 @default.
- W4367054436 creator A5029483764 @default.
- W4367054436 creator A5057573030 @default.
- W4367054436 creator A5058682142 @default.
- W4367054436 creator A5068797103 @default.
- W4367054436 date "2023-04-25" @default.
- W4367054436 modified "2023-10-14" @default.
- W4367054436 title "Positive Effect of Acetylation on Proteomic Analysis Based on Liquid Chromatography with Atmospheric Pressure Chemical Ionization and Photoionization Mass Spectrometry" @default.
- W4367054436 cites W120080674 @default.
- W4367054436 cites W1525575010 @default.
- W4367054436 cites W1620630009 @default.
- W4367054436 cites W1966600210 @default.
- W4367054436 cites W1970969342 @default.
- W4367054436 cites W1973405486 @default.
- W4367054436 cites W1975172955 @default.
- W4367054436 cites W1977168348 @default.
- W4367054436 cites W1978216187 @default.
- W4367054436 cites W1981593008 @default.
- W4367054436 cites W1981934412 @default.
- W4367054436 cites W1982416046 @default.
- W4367054436 cites W1983255121 @default.
- W4367054436 cites W1988812826 @default.
- W4367054436 cites W1990527227 @default.
- W4367054436 cites W1991430603 @default.
- W4367054436 cites W1994711141 @default.
- W4367054436 cites W1996316908 @default.
- W4367054436 cites W1998867917 @default.
- W4367054436 cites W2001160940 @default.
- W4367054436 cites W2005700058 @default.
- W4367054436 cites W2007425565 @default.
- W4367054436 cites W2007520843 @default.
- W4367054436 cites W2009252088 @default.
- W4367054436 cites W2010164543 @default.
- W4367054436 cites W2011646882 @default.
- W4367054436 cites W2013675253 @default.
- W4367054436 cites W2014690911 @default.
- W4367054436 cites W2016674528 @default.
- W4367054436 cites W2016717705 @default.
- W4367054436 cites W2019379138 @default.
- W4367054436 cites W2025004371 @default.
- W4367054436 cites W2028784365 @default.
- W4367054436 cites W2029629076 @default.
- W4367054436 cites W2033171034 @default.
- W4367054436 cites W2033879127 @default.
- W4367054436 cites W2035702956 @default.
- W4367054436 cites W2040308794 @default.
- W4367054436 cites W2040937930 @default.
- W4367054436 cites W2043894952 @default.
- W4367054436 cites W2044039308 @default.
- W4367054436 cites W2046532006 @default.
- W4367054436 cites W2047005630 @default.
- W4367054436 cites W2048346691 @default.
- W4367054436 cites W2051429976 @default.
- W4367054436 cites W2054545863 @default.
- W4367054436 cites W2057855481 @default.
- W4367054436 cites W2063914793 @default.
- W4367054436 cites W2064129161 @default.
- W4367054436 cites W2066387545 @default.
- W4367054436 cites W2072915721 @default.
- W4367054436 cites W2078723663 @default.
- W4367054436 cites W2086680183 @default.
- W4367054436 cites W2087244783 @default.
- W4367054436 cites W2087386675 @default.
- W4367054436 cites W2087991560 @default.
- W4367054436 cites W2088750902 @default.
- W4367054436 cites W2101879802 @default.
- W4367054436 cites W2110413022 @default.
- W4367054436 cites W2111208057 @default.
- W4367054436 cites W2141809233 @default.
- W4367054436 cites W2281865134 @default.
- W4367054436 cites W2324154197 @default.
- W4367054436 cites W2415968471 @default.
- W4367054436 cites W2464719147 @default.
- W4367054436 cites W2508317742 @default.
- W4367054436 cites W2517327512 @default.
- W4367054436 cites W2586139241 @default.
- W4367054436 cites W2592057293 @default.
- W4367054436 cites W2595661769 @default.
- W4367054436 cites W2601772967 @default.
- W4367054436 cites W2734683753 @default.
- W4367054436 cites W2791180853 @default.
- W4367054436 cites W2793559947 @default.
- W4367054436 cites W2955660512 @default.
- W4367054436 cites W2964695182 @default.
- W4367054436 cites W2991603986 @default.
- W4367054436 cites W2998889434 @default.
- W4367054436 cites W3039939852 @default.
- W4367054436 cites W3128942956 @default.
- W4367054436 cites W3150429321 @default.
- W4367054436 cites W4210374696 @default.
- W4367054436 cites W4240988013 @default.
- W4367054436 cites W4304757231 @default.
- W4367054436 cites W4309348236 @default.
- W4367054436 cites W4321186604 @default.
- W4367054436 cites W4367054436 @default.