Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367145074> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4367145074 endingPage "A99" @default.
- W4367145074 startingPage "A99" @default.
- W4367145074 abstract "Ultrasound targeted microbubble cavitation (UTMC) enhances drug-delivery across the endothelial barrier, but the underlying molecular mechanisms require further investigation. Here we explore the role of mechanosensitive piezo1 channels in regulating UTMC-induced hyperpermeability. Human coronary artery endothelial cells on transwells showed that UTMC (1 MHz, 250 kPa, 10 cycles, 10 ms interval for 10 s) caused hyperpermeability, demonstrated by reduced transendothelial electrical resistance (TEER) (1.7-fold, p < 0.05) and increased dextran flux across the monolayer (twofold, p < 0.01 for 70 kDa dextran, 1.6-fold, p < 0.05 for 10 kDa dextran). UTMC enhanced the paracellular permeability as shown by transient increase in inter-endothelial gaps. Inhibition of Ca2+ influx using mechanosensitive channel inhibitor GsMTx4, or piezo1 siRNA abrogated UTMC-induced hyperpermeability. Reduction in Ca2+ influx diminished inter-endothelialgaps and stress-fiber formation, suggesting Ca2+ influx is required, at least in part, for UTMC-induced hyperpermeability. UTMC increased nitric oxide (NO) production and inhibition of endothelial NO synthase with L-NAME abrogated UTMC-induced hyperpermeability, indicating a role for NO. Immunostaining showed that UTMC caused reorganization of adherens-junction protein VE-cadherin from a linear to interrupted pattern, which is known to be associated with hyperpermeability. Further elucidation of these pathways will aid in clinical translation and optimization of UTMC for delivery of cell-impermeant drugs." @default.
- W4367145074 created "2023-04-28" @default.
- W4367145074 creator A5002682675 @default.
- W4367145074 creator A5077714526 @default.
- W4367145074 creator A5081571713 @default.
- W4367145074 date "2023-03-01" @default.
- W4367145074 modified "2023-09-30" @default.
- W4367145074 title "Calcium influx via mechanosensitive piezo1 channels regulates ultrasound targeted microbubble cavitation-induced endothelial hyperpermeability" @default.
- W4367145074 doi "https://doi.org/10.1121/10.0018298" @default.
- W4367145074 hasPublicationYear "2023" @default.
- W4367145074 type Work @default.
- W4367145074 citedByCount "0" @default.
- W4367145074 crossrefType "journal-article" @default.
- W4367145074 hasAuthorship W4367145074A5002682675 @default.
- W4367145074 hasAuthorship W4367145074A5077714526 @default.
- W4367145074 hasAuthorship W4367145074A5081571713 @default.
- W4367145074 hasConcept C120882062 @default.
- W4367145074 hasConcept C12554922 @default.
- W4367145074 hasConcept C126838900 @default.
- W4367145074 hasConcept C143753070 @default.
- W4367145074 hasConcept C170493617 @default.
- W4367145074 hasConcept C185592680 @default.
- W4367145074 hasConcept C2777339698 @default.
- W4367145074 hasConcept C2780785647 @default.
- W4367145074 hasConcept C4001165 @default.
- W4367145074 hasConcept C41625074 @default.
- W4367145074 hasConcept C50254741 @default.
- W4367145074 hasConcept C55493867 @default.
- W4367145074 hasConcept C60903277 @default.
- W4367145074 hasConcept C64012434 @default.
- W4367145074 hasConcept C70007751 @default.
- W4367145074 hasConcept C71924100 @default.
- W4367145074 hasConcept C86803240 @default.
- W4367145074 hasConcept C95444343 @default.
- W4367145074 hasConceptScore W4367145074C120882062 @default.
- W4367145074 hasConceptScore W4367145074C12554922 @default.
- W4367145074 hasConceptScore W4367145074C126838900 @default.
- W4367145074 hasConceptScore W4367145074C143753070 @default.
- W4367145074 hasConceptScore W4367145074C170493617 @default.
- W4367145074 hasConceptScore W4367145074C185592680 @default.
- W4367145074 hasConceptScore W4367145074C2777339698 @default.
- W4367145074 hasConceptScore W4367145074C2780785647 @default.
- W4367145074 hasConceptScore W4367145074C4001165 @default.
- W4367145074 hasConceptScore W4367145074C41625074 @default.
- W4367145074 hasConceptScore W4367145074C50254741 @default.
- W4367145074 hasConceptScore W4367145074C55493867 @default.
- W4367145074 hasConceptScore W4367145074C60903277 @default.
- W4367145074 hasConceptScore W4367145074C64012434 @default.
- W4367145074 hasConceptScore W4367145074C70007751 @default.
- W4367145074 hasConceptScore W4367145074C71924100 @default.
- W4367145074 hasConceptScore W4367145074C86803240 @default.
- W4367145074 hasConceptScore W4367145074C95444343 @default.
- W4367145074 hasIssue "3_supplement" @default.
- W4367145074 hasLocation W43671450741 @default.
- W4367145074 hasOpenAccess W4367145074 @default.
- W4367145074 hasPrimaryLocation W43671450741 @default.
- W4367145074 hasRelatedWork W1972071814 @default.
- W4367145074 hasRelatedWork W2134577432 @default.
- W4367145074 hasRelatedWork W2908301682 @default.
- W4367145074 hasRelatedWork W2910792276 @default.
- W4367145074 hasRelatedWork W2949915099 @default.
- W4367145074 hasRelatedWork W3004460730 @default.
- W4367145074 hasRelatedWork W3122270077 @default.
- W4367145074 hasRelatedWork W3217390172 @default.
- W4367145074 hasRelatedWork W4206164837 @default.
- W4367145074 hasRelatedWork W4210973055 @default.
- W4367145074 hasVolume "153" @default.
- W4367145074 isParatext "false" @default.
- W4367145074 isRetracted "false" @default.
- W4367145074 workType "article" @default.