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- W4367296775 abstract "Abstract Background Phospholipases As ( PLAs ) are acyl hydrolase that can catalyze the release of free fatty acids in phospholipids and play multiple functions in plant growth and development. PLAs can be divided into three families, PLA1 , PLA2 ( sPLA ) and patatin-related PLA ( pPLA ). Previous studies have elucidated the versatile roles of pPLAs in growth and development of many plants. Methods Genome-wide analysis of the pPLA family and screening of genes for expression verification and gene silencing verification. After gene silencing, analysis of pollen expression pattern, pollen vitality test and POD, SOD, CAT, MDA and H 2 O 2 detection. Result In this study, a total of 294 pPLAs were identified from 13 species, including 46 GhpPLAs divided into three subfamilies (I-III-γ). Expression pattern analysis revealed that most of GhpPLAs were preferentially expressed in the reproductive organs (petal, pistil, anther and ovule), especially two genes ( GhpPLA23 and GhpPLA44 ) were deduced that may affect the reproductive development of G. hirsutum . VIGS experiment of GhpPLA23 and GhpPLA44 showed that the pollen activity decreased with their lower expression levels in the silenced plants. The contents of POD, CAT, SOD and other physiological indicators were significantly increased, while ROS and ROS scavengers were significantly changed after genes silencing, indicating that these two genes had important effects on cotton reproductive development. These findings suggest that pPLAs play a crucial role in reproductive development of G. hirsutum and show the potential to be utilized as candidate genes for haploid inducing. Conclusions In this study, pPLA genes play an important role in the reproductive organs of cotton, affecting the development of flower organs and sperm cells, so this family may play an important role in the reproductive development progress of cotton, and even in inducing cotton to produce haploid." @default.
- W4367296775 created "2023-04-29" @default.
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- W4367296775 date "2023-04-28" @default.
- W4367296775 modified "2023-10-17" @default.
- W4367296775 title "Characteristics of pPLA family and their roles in reproductive development of Gossypium hirsutum L" @default.
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- W4367296775 doi "https://doi.org/10.21203/rs.3.rs-2848379/v1" @default.
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