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- W4367302548 endingPage "120016" @default.
- W4367302548 startingPage "120016" @default.
- W4367302548 abstract "Anammox bacteria rely heavily on iron and have many iron storage sites. However, the biological significance of these iron storage sites has not been clearly defined. In this study, we explored the properties and location of iron storage sites to better understand their cellular function. To do this, the Candidatus Kuenenia stuttgartiensis iron storage protein, bacterioferritin (K.S Bfr), was successfully expressed and purified. In vitro, correctly assembled globulins were observed by transmission electron microscopy. The self-assembled K.S Bfr has active redox and can bind Fe2+ and mineralize it in the protein cavity. In vivo, engineered bacteria with K.S Bfr showed good adaptability to Fe2+, with a survival rate of 78.9% when exposed to 5 mM Fe2+, compared with only 66.0% for wild-type bacteria lacking K.S Bfr. A potential iron regulatory strategy similar to that of Anammox was identified in transcriptomic analysis of engineered bacteria. This system may be controlled by the iron uptake regulator Furto transport Fe2+ via FeoB and store excess Fe2+ in K.S Bfr to maintain cellular homeostasis. K.S Bfr has superior iron storage capacity both intracellularly and in vitro. The discovery of K.S Bfr reveals the storage location of iron-rich nanoparticles, increases our understanding of the adaptability of iron-dependent bacteria to Fe2+, and suggests possible iron regulation strategies in Anammox bacteria." @default.
- W4367302548 created "2023-04-29" @default.
- W4367302548 creator A5005439774 @default.
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- W4367302548 creator A5051855860 @default.
- W4367302548 creator A5052029815 @default.
- W4367302548 creator A5067815949 @default.
- W4367302548 date "2023-06-01" @default.
- W4367302548 modified "2023-10-14" @default.
- W4367302548 title "Unraveling the structure and function of bacterioferritin in Candidatus Kuenenia stuttgartiensis: Iron storage sites maintain cellular iron homeostasis" @default.
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- W4367302548 doi "https://doi.org/10.1016/j.watres.2023.120016" @default.
- W4367302548 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37146397" @default.