Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367314024> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4367314024 endingPage "534" @default.
- W4367314024 startingPage "525" @default.
- W4367314024 abstract "In the present study, Phenylalanine ammonia lyase (PAL) activity was detected in different parts of grape plant (leaves, pulp, whole seeds and defatted seeds) extracts. The results revealed that the whole seed possess highest PAL activity (69.44 U), followed by defatted seed (44.22 U), whereas extracts of leaves and pulp gave the lowest activity values (22.77 and 5.11 U, respectively). The optimum conditions for crude PAL extraction achieved using 50mM potassium phosphate buffer (pH 7) at mixing ratios of (1:7) for 1 h. extraction time. PAL was shown to precipitate out at the saturation range between 20-90%. For further purification, gel filtration chromatography using Sephadex G-200 column (1.5×70) and ion exchange chromatography using DEAE- Cellulose (DE 52) column (3.2× 10) were applied. The specific activity, number of folds and yield of purified PAL was 1157 U/mg, 19.94 and 15.32 % respectively." @default.
- W4367314024 created "2023-04-29" @default.
- W4367314024 creator A5009663782 @default.
- W4367314024 creator A5020779285 @default.
- W4367314024 date "2023-04-28" @default.
- W4367314024 modified "2023-09-28" @default.
- W4367314024 title "OPTIMUM CONDITIONS FOR PHENYLALANINE AMMONIA LYASE EXTRACTION AND PURIFICATION FROM LOCALLY CULTIVATED GRAPE SEEDS" @default.
- W4367314024 doi "https://doi.org/10.36103/ijas.v54i2.1728" @default.
- W4367314024 hasPublicationYear "2023" @default.
- W4367314024 type Work @default.
- W4367314024 citedByCount "0" @default.
- W4367314024 crossrefType "journal-article" @default.
- W4367314024 hasAuthorship W4367314024A5009663782 @default.
- W4367314024 hasAuthorship W4367314024A5020779285 @default.
- W4367314024 hasBestOaLocation W43673140241 @default.
- W4367314024 hasConcept C142724271 @default.
- W4367314024 hasConcept C163994747 @default.
- W4367314024 hasConcept C172854840 @default.
- W4367314024 hasConcept C181199279 @default.
- W4367314024 hasConcept C185592680 @default.
- W4367314024 hasConcept C188442228 @default.
- W4367314024 hasConcept C193363816 @default.
- W4367314024 hasConcept C2776382133 @default.
- W4367314024 hasConcept C2777132085 @default.
- W4367314024 hasConcept C2777191336 @default.
- W4367314024 hasConcept C2777431362 @default.
- W4367314024 hasConcept C2778112864 @default.
- W4367314024 hasConcept C2779251873 @default.
- W4367314024 hasConcept C43617362 @default.
- W4367314024 hasConcept C4725764 @default.
- W4367314024 hasConcept C515207424 @default.
- W4367314024 hasConcept C51724486 @default.
- W4367314024 hasConcept C55493867 @default.
- W4367314024 hasConcept C71924100 @default.
- W4367314024 hasConceptScore W4367314024C142724271 @default.
- W4367314024 hasConceptScore W4367314024C163994747 @default.
- W4367314024 hasConceptScore W4367314024C172854840 @default.
- W4367314024 hasConceptScore W4367314024C181199279 @default.
- W4367314024 hasConceptScore W4367314024C185592680 @default.
- W4367314024 hasConceptScore W4367314024C188442228 @default.
- W4367314024 hasConceptScore W4367314024C193363816 @default.
- W4367314024 hasConceptScore W4367314024C2776382133 @default.
- W4367314024 hasConceptScore W4367314024C2777132085 @default.
- W4367314024 hasConceptScore W4367314024C2777191336 @default.
- W4367314024 hasConceptScore W4367314024C2777431362 @default.
- W4367314024 hasConceptScore W4367314024C2778112864 @default.
- W4367314024 hasConceptScore W4367314024C2779251873 @default.
- W4367314024 hasConceptScore W4367314024C43617362 @default.
- W4367314024 hasConceptScore W4367314024C4725764 @default.
- W4367314024 hasConceptScore W4367314024C515207424 @default.
- W4367314024 hasConceptScore W4367314024C51724486 @default.
- W4367314024 hasConceptScore W4367314024C55493867 @default.
- W4367314024 hasConceptScore W4367314024C71924100 @default.
- W4367314024 hasIssue "2" @default.
- W4367314024 hasLocation W43673140241 @default.
- W4367314024 hasOpenAccess W4367314024 @default.
- W4367314024 hasPrimaryLocation W43673140241 @default.
- W4367314024 hasRelatedWork W1972141502 @default.
- W4367314024 hasRelatedWork W1989594401 @default.
- W4367314024 hasRelatedWork W2050733347 @default.
- W4367314024 hasRelatedWork W2074263550 @default.
- W4367314024 hasRelatedWork W2094167934 @default.
- W4367314024 hasRelatedWork W2371639062 @default.
- W4367314024 hasRelatedWork W2408302217 @default.
- W4367314024 hasRelatedWork W2408954907 @default.
- W4367314024 hasRelatedWork W4367314024 @default.
- W4367314024 hasRelatedWork W801973253 @default.
- W4367314024 hasVolume "54" @default.
- W4367314024 isParatext "false" @default.
- W4367314024 isRetracted "false" @default.
- W4367314024 workType "article" @default.