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• W4367314712 abstract "Theodoxus fluviatilis (Linnaeus, 1758) (Gastropoda: Neritidae) is an oligohaline aquatic gastropod that inhabits most of Europe and adjacent areas of Asia. Two different ecotypes can be distinguished: One in freshwater (FW) and another along the Baltic Sea coast in brackish water habitats (BW). Individuals of either ecotype use free amino acids and urea as organic osmolytes to adjust body fluid osmolality to the external medium; however, the BW ecotype is able to accumulate them in larger quantities. The use of urea as an organic osmolyte in aquatic gastropods such as T. fluviatilis has only recently been initially described and raised the question of how urea transport between body fluids and the environment is balanced. Upon examining transcriptome and preliminary genome sequence data of T. fluviatilis, we identified putative homologues of DUR3 genes, which code for urea transporters (UTs) in other organisms. In this study, we provide evidence for the presence of four different subtypes of DUR3-like UTs that belong to two distinct families. Two of the UT subtypes were subject to qRT-PCR analyses to investigate differences in mRNA expression during the acclimation of individuals of both ecotypes to different salinities. Our results indicate that only BW animals regulate DUR3 gene expression in the context of osmoregulation." @default.
• W4367314712 created "2023-04-29" @default.
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• W4367314712 date "2023-04-28" @default.
• W4367314712 modified "2023-09-30" @default.
• W4367314712 title "The Ins and Outs of Urea: Identification of Putative DUR3-like Urea Transporters in the Oligohaline Nerite Snail Theodoxus fluviatilis and Their Expression under Changing Salinities" @default.
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• W4367314712 doi "https://doi.org/10.3390/physiologia3020020" @default.
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