FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367395044> ?p ?o ?g. }

• W4367395044 endingPage "257" @default.
• W4367395044 startingPage "237" @default.
• W4367395044 abstract "Phosphorylated biomarkers are crucial for our understanding of drug mechanism of action and dose selection during clinical trials, particularly for drugs that target protein kinases, such as DNA-damage-response (DDR) inhibitors. However, tissue fixation conditions needed to preserve DDR-specific phospho-biomarkers have not been previously investigated. Using xenograft tissues and tightly controlled formalin fixation conditions, we assessed how preanalytical factors affect phosphorylated DDR biomarkers pRAD50(Ser635), ɣH2AX(Ser139), pKAP1(Ser824), and non-phosphorylated biomarkers cMYC and ATM. Cold ischemia times ranged from 15 min to 6 hr, and the fixation duration ranged from 24 hr to 4 weeks. Epitopes pRAD50 and pKAP1 appeared the most labile assessed with staining loss after just 15 min of cold ischemia time, while ATM was more robust showing consistent expression up to 1 hr of cold ischemia. Notably, ɣH2AX expression was lost with formalin fixation over 48 hr. The use of core needle biopsies where possible and novel fixation methods such as the 2-step temperature-controlled formalin approach may improve phosphorylated biomarker preservation; however, practical challenges may affect wider clinical application. The most essential tissue-processing step when downstream analysis includes DDR phosphorylated biomarkers is immediate tissue submersion in formalin, without delay, upon excision from the patient, followed by room temperature fixation for 24 hr." @default.
• W4367395044 created "2023-04-30" @default.
• W4367395044 creator A5020083935 @default.
• W4367395044 creator A5022677387 @default.
• W4367395044 creator A5043391366 @default.
• W4367395044 creator A5043935669 @default.
• W4367395044 creator A5045151645 @default.
• W4367395044 creator A5053829932 @default.
• W4367395044 creator A5059049926 @default.
• W4367395044 creator A5076715584 @default.
• W4367395044 creator A5091570131 @default.
• W4367395044 date "2023-04-29" @default.
• W4367395044 modified "2023-09-30" @default.
• W4367395044 title "Epitope Lability of Phosphorylated Biomarkers of the DNA Damage Response Pathway Results in Increased Vulnerability to Effects of Delayed or Incomplete Formalin Fixation" @default.
• W4367395044 cites W1881198168 @default.
• W4367395044 cites W1976672681 @default.
• W4367395044 cites W1980529614 @default.
• W4367395044 cites W1994702880 @default.
• W4367395044 cites W1998512193 @default.
• W4367395044 cites W2010257344 @default.
• W4367395044 cites W2026145190 @default.
• W4367395044 cites W2046913227 @default.
• W4367395044 cites W2048142647 @default.
• W4367395044 cites W2054389173 @default.
• W4367395044 cites W2073668315 @default.
• W4367395044 cites W2091291785 @default.
• W4367395044 cites W2094697116 @default.
• W4367395044 cites W2096441602 @default.
• W4367395044 cites W2108776415 @default.
• W4367395044 cites W2121404525 @default.
• W4367395044 cites W2134000127 @default.
• W4367395044 cites W2136859769 @default.
• W4367395044 cites W2158997529 @default.
• W4367395044 cites W2414367793 @default.
• W4367395044 cites W2536593745 @default.
• W4367395044 cites W2635415917 @default.
• W4367395044 cites W2773498353 @default.
• W4367395044 cites W2790561936 @default.
• W4367395044 cites W2791177608 @default.
• W4367395044 cites W2899040421 @default.
• W4367395044 cites W2954933200 @default.
• W4367395044 cites W3005380101 @default.
• W4367395044 cites W3032665916 @default.
• W4367395044 cites W4231018668 @default.
• W4367395044 cites W4234081105 @default.
• W4367395044 cites W4249815350 @default.
• W4367395044 doi "https://doi.org/10.1369/00221554231174069" @default.
• W4367395044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37119278" @default.
• W4367395044 hasPublicationYear "2023" @default.
• W4367395044 type Work @default.
• W4367395044 citedByCount "0" @default.
• W4367395044 crossrefType "journal-article" @default.
• W4367395044 hasAuthorship W4367395044A5020083935 @default.
• W4367395044 hasAuthorship W4367395044A5022677387 @default.
• W4367395044 hasAuthorship W4367395044A5043391366 @default.
• W4367395044 hasAuthorship W4367395044A5043935669 @default.
• W4367395044 hasAuthorship W4367395044A5045151645 @default.
• W4367395044 hasAuthorship W4367395044A5053829932 @default.
• W4367395044 hasAuthorship W4367395044A5059049926 @default.
• W4367395044 hasAuthorship W4367395044A5076715584 @default.
• W4367395044 hasAuthorship W4367395044A5091570131 @default.
• W4367395044 hasConcept C104317684 @default.
• W4367395044 hasConcept C11960822 @default.
• W4367395044 hasConcept C142724271 @default.
• W4367395044 hasConcept C143425029 @default.
• W4367395044 hasConcept C146249460 @default.
• W4367395044 hasConcept C184235292 @default.
• W4367395044 hasConcept C2781197716 @default.
• W4367395044 hasConcept C502942594 @default.
• W4367395044 hasConcept C552990157 @default.
• W4367395044 hasConcept C55493867 @default.
• W4367395044 hasConcept C71924100 @default.
• W4367395044 hasConcept C86803240 @default.
• W4367395044 hasConcept C95444343 @default.
• W4367395044 hasConceptScore W4367395044C104317684 @default.
• W4367395044 hasConceptScore W4367395044C11960822 @default.
• W4367395044 hasConceptScore W4367395044C142724271 @default.
• W4367395044 hasConceptScore W4367395044C143425029 @default.
• W4367395044 hasConceptScore W4367395044C146249460 @default.
• W4367395044 hasConceptScore W4367395044C184235292 @default.
• W4367395044 hasConceptScore W4367395044C2781197716 @default.
• W4367395044 hasConceptScore W4367395044C502942594 @default.
• W4367395044 hasConceptScore W4367395044C552990157 @default.
• W4367395044 hasConceptScore W4367395044C55493867 @default.
• W4367395044 hasConceptScore W4367395044C71924100 @default.
• W4367395044 hasConceptScore W4367395044C86803240 @default.
• W4367395044 hasConceptScore W4367395044C95444343 @default.
• W4367395044 hasIssue "5" @default.
• W4367395044 hasLocation W43673950441 @default.
• W4367395044 hasLocation W43673950442 @default.
• W4367395044 hasOpenAccess W4367395044 @default.
• W4367395044 hasPrimaryLocation W43673950441 @default.
• W4367395044 hasRelatedWork W1494348311 @default.
• W4367395044 hasRelatedWork W2005284058 @default.
• W4367395044 hasRelatedWork W2012362589 @default.
• W4367395044 hasRelatedWork W2015725807 @default.
• W4367395044 hasRelatedWork W2050981751 @default.
• W4367395044 hasRelatedWork W2064840560 @default.

• next