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- W4367668869 abstract "The sensitive and accurate detection of biomarkers plays an important role in clinical diagnosis and drug discovery. Currently, amplification-based methods for biomarker detection are widely explored. However, the key challenges of these methods are limited reproducibility and high background noise. To overcome these limitations, we develop a robust plasmonic nanoparticle-coupled single-molecule kinetic fingerprinting (PNP-SMKF) method to achieve ultrasensitive detection of protein kinase A (PKA). Transient binding of a short fluorescent probe with the genuine target produces a distinct kinetic signature that is completely different from that of the background signal, allowing us to recognize PKA sensitively. Importantly, integrating a plasmonic nanoparticle efficiently breaks the concentration limit of the imager strand for single-molecule imaging, thus achieving a much faster imaging speed. A limit of detection (LOD) of as low as 0.0005 U/mL is readily realized. This method holds great potential as a versatile platform for enzyme detection and inhibitor screening in the future." @default.
- W4367668869 created "2023-05-03" @default.
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- W4367668869 date "2023-05-02" @default.
- W4367668869 modified "2023-10-07" @default.
- W4367668869 title "Nanoparticle-Coupled Single-Molecule Kinetic Fingerprinting for Enzymatic Activity Detection" @default.
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- W4367668869 doi "https://doi.org/10.1021/acs.analchem.3c01385" @default.
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