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• W4367669274 endingPage "7684" @default.
• W4367669274 startingPage "7676" @default.
• W4367669274 abstract "Herein, we report a fluorescence strategy for the homogeneous and simultaneous analysis of urine miRNA-375 and miRNA-148a. The target miRNAs in urine bonded the devised dumbbell-shaped “C-Ag+-C” and “T-Hg2+-T” hairpin structures that could trigger cascade enzyme-free amplification. Then, the fluorescent CdTe quantum dots (QDs) and carbon dots (CDs) could selectively recognize Ag+ and Hg2+, to quantify the dual miRNAs concurrently. Under optimized conditions, the linear range was from 0.1 to 1000 fM and the limits of detection (LOD) for dual miRNAs reached 30 and 25 aM, respectively. The practicality was further evaluated with 45 clinical urine samples including prostate cancer (PC) and other patients, and the results were consistent with the clinical polymerase chain reaction (PCR) kit and ultrasonic and pathological findings. The receiver operating characteristic (ROC) curve analysis showed that the estimates of the area under the curve (AUC) were 0.739 for the serum prostate-specific antigen (PSA) and 0.941 for miRNA-375 and 0.946 for miRNA-148a. The sensitivity and specificity reached 75 and 100% for miRNA-375 and 71 and 94% for miRNA-148a, respectively, which was better than serum PSA. This strategy constructed a reliable system for dual miRNA detection in urine samples and proposed new insights into the rapid and noninvasive diagnosis of PC." @default.
• W4367669274 created "2023-05-03" @default.
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• W4367669274 date "2023-05-02" @default.
• W4367669274 modified "2023-10-14" @default.
• W4367669274 title "Nanoarchitectonics-Assisted Simultaneous Fluorescence Detection of Urinary Dual miRNAs for Noninvasive Diagnosis of Prostate Cancer" @default.
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• W4367669274 doi "https://doi.org/10.1021/acs.analchem.3c00701" @default.
• W4367669274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37129316" @default.
• W4367669274 hasPublicationYear "2023" @default.
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