FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367678832> ?p ?o ?g. }

• W4367678832 abstract "Notch signaling is critical for regulating the function of vascular endothelial cells (ECs). However, the effect of the intracellular domain of Notch1 (NICD) on EC injury in sepsis remains unclear.We established a cell model of vascular endothelial dysfunction and induced sepsis in a mouse model via lipopolysaccharide (LPS) injection and cecal ligation and puncture (CLP). Endothelial barrier function and expression of endothelial-related proteins were determined using CCK-8, permeability, flow cytometry, immunoblot, and immunoprecipitation assays. The effect of NICD inhibition or activation on endothelial barrier function was evaluated in vitro. Melatonin was used for NICD activation in sepsis mice. The survival rate, Evans blue dye of organs, vessel relaxation assay, immunohistochemistry, ELISA, immunoblot were used to explore the specific role of melatonin for sepsis induced vascular dysfunction in vivo.We found that LPS, interleukin 6, and serum collected from septic children could inhibit the expression of NICD and its downstream regulator Hes1, which impaired endothelial barrier function and led to EC apoptosis through the AKT pathway. Mechanistically, LPS decreased the stability of NICD by inhibiting the expression of a deubiquitylating enzyme, ubiquitin-specific proteases 8 (USP8). Melatonin, however, upregulated USP8 expression, thus maintaining the stability of NICD and Notch signaling, which ultimately reduced EC injury in our sepsis model and elevated the survival rate of septic mice.We found a previously uncharacterized role of Notch1 in mediating vascular permeability during sepsis, and we showed that inhibition of NICD resulted in vascular EC dysfunction in sepsis, which was reversed by melatonin. Thus, the Notch1 signaling pathway is a potential target for the treatment of sepsis." @default.
• W4367678832 created "2023-05-03" @default.
• W4367678832 creator A5000894411 @default.
• W4367678832 creator A5005776144 @default.
• W4367678832 creator A5024671936 @default.
• W4367678832 creator A5072129534 @default.
• W4367678832 creator A5074660144 @default.
• W4367678832 creator A5085506535 @default.
• W4367678832 creator A5088551712 @default.
• W4367678832 date "2023-05-02" @default.
• W4367678832 modified "2023-09-30" @default.
• W4367678832 title "Inhibition of the intracellular domain of Notch1 results in vascular endothelial cell dysfunction in sepsis" @default.
• W4367678832 cites W1555109275 @default.
• W4367678832 cites W1926960484 @default.
• W4367678832 cites W1964461246 @default.
• W4367678832 cites W1971571526 @default.
• W4367678832 cites W1990527911 @default.
• W4367678832 cites W1991797784 @default.
• W4367678832 cites W1995068520 @default.
• W4367678832 cites W2015087171 @default.
• W4367678832 cites W2023945291 @default.
• W4367678832 cites W2061996746 @default.
• W4367678832 cites W2091365164 @default.
• W4367678832 cites W2118439228 @default.
• W4367678832 cites W2119541227 @default.
• W4367678832 cites W2129261740 @default.
• W4367678832 cites W2148915576 @default.
• W4367678832 cites W2154562194 @default.
• W4367678832 cites W2168438265 @default.
• W4367678832 cites W2176735297 @default.
• W4367678832 cites W2255691977 @default.
• W4367678832 cites W2504255207 @default.
• W4367678832 cites W2518763897 @default.
• W4367678832 cites W2602661209 @default.
• W4367678832 cites W2620241325 @default.
• W4367678832 cites W2768469522 @default.
• W4367678832 cites W2770671163 @default.
• W4367678832 cites W2773024908 @default.
• W4367678832 cites W2788052557 @default.
• W4367678832 cites W2799445034 @default.
• W4367678832 cites W2803015713 @default.
• W4367678832 cites W2808829764 @default.
• W4367678832 cites W2942005780 @default.
• W4367678832 cites W2971534592 @default.
• W4367678832 cites W2981836840 @default.
• W4367678832 cites W2998853022 @default.
• W4367678832 cites W3004427938 @default.
• W4367678832 cites W3005194773 @default.
• W4367678832 cites W3005429114 @default.
• W4367678832 cites W3009049647 @default.
• W4367678832 cites W3018293666 @default.
• W4367678832 cites W3080714364 @default.
• W4367678832 cites W3087558244 @default.
• W4367678832 cites W3093246528 @default.
• W4367678832 cites W3157343119 @default.
• W4367678832 cites W4282919767 @default.
• W4367678832 cites W4285159704 @default.
• W4367678832 cites W4285592537 @default.
• W4367678832 doi "https://doi.org/10.3389/fimmu.2023.1134556" @default.
• W4367678832 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37205094" @default.
• W4367678832 hasPublicationYear "2023" @default.
• W4367678832 type Work @default.
• W4367678832 citedByCount "2" @default.
• W4367678832 countsByYear W43676788322023 @default.
• W4367678832 crossrefType "journal-article" @default.
• W4367678832 hasAuthorship W4367678832A5000894411 @default.
• W4367678832 hasAuthorship W4367678832A5005776144 @default.
• W4367678832 hasAuthorship W4367678832A5024671936 @default.
• W4367678832 hasAuthorship W4367678832A5072129534 @default.
• W4367678832 hasAuthorship W4367678832A5074660144 @default.
• W4367678832 hasAuthorship W4367678832A5085506535 @default.
• W4367678832 hasAuthorship W4367678832A5088551712 @default.
• W4367678832 hasBestOaLocation W43676788321 @default.
• W4367678832 hasConcept C104317684 @default.
• W4367678832 hasConcept C113045295 @default.
• W4367678832 hasConcept C123012128 @default.
• W4367678832 hasConcept C126322002 @default.
• W4367678832 hasConcept C127561419 @default.
• W4367678832 hasConcept C134018914 @default.
• W4367678832 hasConcept C161879069 @default.
• W4367678832 hasConcept C185592680 @default.
• W4367678832 hasConcept C202751555 @default.
• W4367678832 hasConcept C203014093 @default.
• W4367678832 hasConcept C2777649898 @default.
• W4367678832 hasConcept C2778384902 @default.
• W4367678832 hasConcept C2780972559 @default.
• W4367678832 hasConcept C502942594 @default.
• W4367678832 hasConcept C55493867 @default.
• W4367678832 hasConcept C62478195 @default.
• W4367678832 hasConcept C71924100 @default.
• W4367678832 hasConcept C86803240 @default.
• W4367678832 hasConcept C95444343 @default.
• W4367678832 hasConceptScore W4367678832C104317684 @default.
• W4367678832 hasConceptScore W4367678832C113045295 @default.
• W4367678832 hasConceptScore W4367678832C123012128 @default.
• W4367678832 hasConceptScore W4367678832C126322002 @default.
• W4367678832 hasConceptScore W4367678832C127561419 @default.
• W4367678832 hasConceptScore W4367678832C134018914 @default.
• W4367678832 hasConceptScore W4367678832C161879069 @default.
• W4367678832 hasConceptScore W4367678832C185592680 @default.

• next