Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367679076> ?p ?o ?g. }
- W4367679076 abstract "Bacteria encode hundreds of diverse defense systems that protect from viral infection and inhibit phage propagation 1–5 . Gabija is one of the most prevalent anti-phage defense systems, occurring in >15% of all sequenced bacterial and archaeal genomes 1,6,7 , but the molecular basis of how Gabija defends cells from viral infection remains poorly understood. Here we use X-ray crystallography and cryo-EM to define how Gabija proteins assemble into an ∼500 kDa supramolecular complex that degrades phage DNA. Gabija protein A (GajA) is a DNA endonuclease that tetramerizes to form the core of the anti-phage defense complex. Two sets of Gabija protein B (GajB) dimers dock at opposite sides of the complex and create a 4:4 GajAB assembly that is essential for phage resistance in vivo . We show that a phage-encoded protein Gabija anti-defense 1 (Gad1) directly binds the Gabija GajAB complex and inactivates defense. A cryo-EM structure of the virally inhibited state reveals that Gad1 forms an octameric web that encases the GajAB complex and inhibits DNA recognition and cleavage. Our results reveal the structural basis of assembly of the Gabija anti-phage defense complex and define a unique mechanism of viral immune evasion." @default.
- W4367679076 created "2023-05-03" @default.
- W4367679076 creator A5007997997 @default.
- W4367679076 creator A5014141606 @default.
- W4367679076 creator A5022647229 @default.
- W4367679076 creator A5024718393 @default.
- W4367679076 creator A5027299558 @default.
- W4367679076 creator A5045303182 @default.
- W4367679076 creator A5048714137 @default.
- W4367679076 creator A5064061778 @default.
- W4367679076 creator A5091376183 @default.
- W4367679076 date "2023-05-01" @default.
- W4367679076 modified "2023-10-14" @default.
- W4367679076 title "Structural basis of Gabija anti-phage defense and viral immune evasion" @default.
- W4367679076 cites W1980002594 @default.
- W4367679076 cites W1993783912 @default.
- W4367679076 cites W1996984611 @default.
- W4367679076 cites W2010708494 @default.
- W4367679076 cites W2030632688 @default.
- W4367679076 cites W2041711910 @default.
- W4367679076 cites W2043083543 @default.
- W4367679076 cites W2051848082 @default.
- W4367679076 cites W2056367986 @default.
- W4367679076 cites W2072690167 @default.
- W4367679076 cites W2098891503 @default.
- W4367679076 cites W2115062176 @default.
- W4367679076 cites W2121453804 @default.
- W4367679076 cites W2144081223 @default.
- W4367679076 cites W2151541959 @default.
- W4367679076 cites W2346708074 @default.
- W4367679076 cites W2397580070 @default.
- W4367679076 cites W2587625522 @default.
- W4367679076 cites W2784532673 @default.
- W4367679076 cites W2884661296 @default.
- W4367679076 cites W2890107400 @default.
- W4367679076 cites W2921875691 @default.
- W4367679076 cites W2964762483 @default.
- W4367679076 cites W2965856049 @default.
- W4367679076 cites W2973457155 @default.
- W4367679076 cites W2999052663 @default.
- W4367679076 cites W2999639869 @default.
- W4367679076 cites W3003219583 @default.
- W4367679076 cites W3081228941 @default.
- W4367679076 cites W3083406432 @default.
- W4367679076 cites W3156568412 @default.
- W4367679076 cites W3177828909 @default.
- W4367679076 cites W4211178931 @default.
- W4367679076 cites W4220957461 @default.
- W4367679076 cites W4220997118 @default.
- W4367679076 cites W4223506823 @default.
- W4367679076 cites W4229443239 @default.
- W4367679076 cites W4280501739 @default.
- W4367679076 cites W4281790889 @default.
- W4367679076 cites W4282913389 @default.
- W4367679076 cites W4286007946 @default.
- W4367679076 cites W4291020693 @default.
- W4367679076 cites W4292415330 @default.
- W4367679076 cites W4296381497 @default.
- W4367679076 cites W4297996539 @default.
- W4367679076 cites W4307493886 @default.
- W4367679076 cites W4311931795 @default.
- W4367679076 cites W4319310891 @default.
- W4367679076 cites W4319728599 @default.
- W4367679076 cites W4322495102 @default.
- W4367679076 doi "https://doi.org/10.1101/2023.05.01.538945" @default.
- W4367679076 hasPublicationYear "2023" @default.
- W4367679076 type Work @default.
- W4367679076 citedByCount "3" @default.
- W4367679076 countsByYear W43676790762023 @default.
- W4367679076 crossrefType "posted-content" @default.
- W4367679076 hasAuthorship W4367679076A5007997997 @default.
- W4367679076 hasAuthorship W4367679076A5014141606 @default.
- W4367679076 hasAuthorship W4367679076A5022647229 @default.
- W4367679076 hasAuthorship W4367679076A5024718393 @default.
- W4367679076 hasAuthorship W4367679076A5027299558 @default.
- W4367679076 hasAuthorship W4367679076A5045303182 @default.
- W4367679076 hasAuthorship W4367679076A5048714137 @default.
- W4367679076 hasAuthorship W4367679076A5064061778 @default.
- W4367679076 hasAuthorship W4367679076A5091376183 @default.
- W4367679076 hasBestOaLocation W43676790761 @default.
- W4367679076 hasConcept C10079403 @default.
- W4367679076 hasConcept C104317684 @default.
- W4367679076 hasConcept C141231307 @default.
- W4367679076 hasConcept C159047783 @default.
- W4367679076 hasConcept C159654299 @default.
- W4367679076 hasConcept C186268636 @default.
- W4367679076 hasConcept C191120209 @default.
- W4367679076 hasConcept C2522874641 @default.
- W4367679076 hasConcept C2776441376 @default.
- W4367679076 hasConcept C2780879997 @default.
- W4367679076 hasConcept C54355233 @default.
- W4367679076 hasConcept C547475151 @default.
- W4367679076 hasConcept C552990157 @default.
- W4367679076 hasConcept C70721500 @default.
- W4367679076 hasConcept C86803240 @default.
- W4367679076 hasConcept C95444343 @default.
- W4367679076 hasConceptScore W4367679076C10079403 @default.
- W4367679076 hasConceptScore W4367679076C104317684 @default.
- W4367679076 hasConceptScore W4367679076C141231307 @default.
- W4367679076 hasConceptScore W4367679076C159047783 @default.