Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367834887> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W4367834887 endingPage "569" @default.
- W4367834887 startingPage "557" @default.
- W4367834887 abstract "Luminex single antigen bead (SAB) kits from One Lambda (OL) and Lifecodes (LC) are widely used for HLA antibody detection but have substantial differences in design and assay protocol resulting in different mean fluorescence intensity (MFI) values. Here, we present a non-linear modeling approach to accurately convert MFI values between two vendors and to establish user-independent MFI cutoffs when analyzing big datasets. HLA antibody data from a total of 47 EDTA-treated sera tested using both OL and LC SAB kits were analyzed. MFI comparisons were made for the common 84 HLA class I and 63 class II beads. In the exploration set (n = 24), a non-linear hyperbola model on raw MFI corrected by locus-specific highest self MFI subtraction yielded the highest correlation (class I r2 : 0.946, class II r2 : 0.898). Performance of the model was verified in an independent validation set (n = 12) (class I r2 : 0.952, class II r2 : 0.911). Furthermore, in an independent cohort of post-transplant serum samples (n = 11) using the vendor-specific MFI cutoffs dictated by the current model, we found 94% accuracy in bead-specific reactivity assignments by the two vendors. We recommend using the non-linear hyperbola modeling approach with self HLA correction and locus-specific analyzes to harmonize MFI values between two vendors in particular research datasets. As there are considerable variations between the two assays, using MFI conversion for individual patient samples is not recommended." @default.
- W4367834887 created "2023-05-04" @default.
- W4367834887 creator A5017033909 @default.
- W4367834887 creator A5019013671 @default.
- W4367834887 creator A5021544227 @default.
- W4367834887 creator A5050563616 @default.
- W4367834887 creator A5080347870 @default.
- W4367834887 creator A5089968075 @default.
- W4367834887 date "2023-05-02" @default.
- W4367834887 modified "2023-10-17" @default.
- W4367834887 title "A modeling approach for mean fluorescence intensity value harmonization and cutoff prediction for luminex single antigen bead assays of two different vendors" @default.
- W4367834887 cites W1527097930 @default.
- W4367834887 cites W2015800825 @default.
- W4367834887 cites W2032850621 @default.
- W4367834887 cites W2033397780 @default.
- W4367834887 cites W2047292563 @default.
- W4367834887 cites W2055442253 @default.
- W4367834887 cites W2097115382 @default.
- W4367834887 cites W2520913131 @default.
- W4367834887 cites W2588800841 @default.
- W4367834887 cites W2618762746 @default.
- W4367834887 cites W2763212433 @default.
- W4367834887 cites W2794595056 @default.
- W4367834887 cites W2794831482 @default.
- W4367834887 cites W2811482705 @default.
- W4367834887 cites W2886498718 @default.
- W4367834887 cites W2887206724 @default.
- W4367834887 cites W2891620783 @default.
- W4367834887 cites W2897708460 @default.
- W4367834887 cites W2948264974 @default.
- W4367834887 cites W2964408868 @default.
- W4367834887 cites W3016713957 @default.
- W4367834887 cites W3017885345 @default.
- W4367834887 cites W3018173909 @default.
- W4367834887 cites W3091814290 @default.
- W4367834887 cites W3176357146 @default.
- W4367834887 cites W4213227004 @default.
- W4367834887 doi "https://doi.org/10.1111/tan.15082" @default.
- W4367834887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37130801" @default.
- W4367834887 hasPublicationYear "2023" @default.
- W4367834887 type Work @default.
- W4367834887 citedByCount "0" @default.
- W4367834887 crossrefType "journal-article" @default.
- W4367834887 hasAuthorship W4367834887A5017033909 @default.
- W4367834887 hasAuthorship W4367834887A5019013671 @default.
- W4367834887 hasAuthorship W4367834887A5021544227 @default.
- W4367834887 hasAuthorship W4367834887A5050563616 @default.
- W4367834887 hasAuthorship W4367834887A5080347870 @default.
- W4367834887 hasAuthorship W4367834887A5089968075 @default.
- W4367834887 hasBestOaLocation W43678348871 @default.
- W4367834887 hasConcept C105795698 @default.
- W4367834887 hasConcept C114614502 @default.
- W4367834887 hasConcept C121332964 @default.
- W4367834887 hasConcept C147483822 @default.
- W4367834887 hasConcept C156340839 @default.
- W4367834887 hasConcept C185592680 @default.
- W4367834887 hasConcept C188280979 @default.
- W4367834887 hasConcept C203014093 @default.
- W4367834887 hasConcept C2778217198 @default.
- W4367834887 hasConcept C33923547 @default.
- W4367834887 hasConcept C41008148 @default.
- W4367834887 hasConcept C43617362 @default.
- W4367834887 hasConcept C62520636 @default.
- W4367834887 hasConcept C71924100 @default.
- W4367834887 hasConceptScore W4367834887C105795698 @default.
- W4367834887 hasConceptScore W4367834887C114614502 @default.
- W4367834887 hasConceptScore W4367834887C121332964 @default.
- W4367834887 hasConceptScore W4367834887C147483822 @default.
- W4367834887 hasConceptScore W4367834887C156340839 @default.
- W4367834887 hasConceptScore W4367834887C185592680 @default.
- W4367834887 hasConceptScore W4367834887C188280979 @default.
- W4367834887 hasConceptScore W4367834887C203014093 @default.
- W4367834887 hasConceptScore W4367834887C2778217198 @default.
- W4367834887 hasConceptScore W4367834887C33923547 @default.
- W4367834887 hasConceptScore W4367834887C41008148 @default.
- W4367834887 hasConceptScore W4367834887C43617362 @default.
- W4367834887 hasConceptScore W4367834887C62520636 @default.
- W4367834887 hasConceptScore W4367834887C71924100 @default.
- W4367834887 hasIssue "5" @default.
- W4367834887 hasLocation W43678348871 @default.
- W4367834887 hasLocation W43678348872 @default.
- W4367834887 hasOpenAccess W4367834887 @default.
- W4367834887 hasPrimaryLocation W43678348871 @default.
- W4367834887 hasRelatedWork W108845024 @default.
- W4367834887 hasRelatedWork W2013740965 @default.
- W4367834887 hasRelatedWork W2026779193 @default.
- W4367834887 hasRelatedWork W2143566611 @default.
- W4367834887 hasRelatedWork W2578985712 @default.
- W4367834887 hasRelatedWork W2952598754 @default.
- W4367834887 hasRelatedWork W4300125793 @default.
- W4367834887 hasRelatedWork W57712237 @default.
- W4367834887 hasRelatedWork W588267724 @default.
- W4367834887 hasRelatedWork W2406961474 @default.
- W4367834887 hasVolume "102" @default.
- W4367834887 isParatext "false" @default.
- W4367834887 isRetracted "false" @default.
- W4367834887 workType "article" @default.