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- W4367840545 abstract "The dinoflagellate Karenia brevis, blooms annually in the Gulf of Mexico, producing a suite of neurotoxins known as the brevetoxins. The cellular toxin content of K. brevis, however, is highly variable between or even within strains. I investigated biochemical differences between high (KbHT) and low (KbLT) toxin producing cultures both derived from the Wilson strain, related to energy-dependent quenching (qE) by photosystem II, and the content of reduced thiols of the proteome. By characterizing the xanthophyll content of the two strains I was able to determine that KbLT performs qE inconsistently. To investigate the source of the differences in qE, RT-qPCR was utilized to examine gene expression of the xanthophyll cycle enzyme diadinoxanthin de-epoxidase (Dde), however no differences in expression were found. Furthermore, using redox proteomics the protein expression of Dde and monogalactosyldiacylglycerol (MGDG) vii synthase were determined to not be significantly different in the two cultures. Also reported are significant differences in the lipidomes of KbHT and KbLT with respect to MGDG, which facilitates the xanthophyll cycle. Redox proteomics experiments detected a significantly higher proportion of proteinogenic cysteine thiols in the reduced thiol state in the low toxin proteome, including plastid localized thioredoxin (Trx), which can result in inactivation of Dde and activation of MGDG synthase. Moreover, recombinant K. brevis thioredoxin reductase (KbTrxR) was produced in order to characterize the interaction of this enzyme with brevetoxin. Brevetoxin was found to inhibit reductase activity towards various substrates. Using mass spectrometry, I was able to detect an adduct of KbTrxR and brevetoxin on a cysteine residue at the N-terminal redox center. This supports the hypothesis that brevetoxin could mediate redox homeostasis by interacting with thiol-disulfide centers in thioredoxin reductase" @default.
- W4367840545 created "2023-05-04" @default.
- W4367840545 creator A5027859974 @default.
- W4367840545 date "2023-05-03" @default.
- W4367840545 modified "2023-09-27" @default.
- W4367840545 title "High and Low Toxin Producing Strains of Karenia Brevis Differ Significantly in the Redox Proteome, Lipid Profiles, and Xanthophyll Cycle Pigments" @default.
- W4367840545 doi "https://doi.org/10.25148/etd.fidc010220" @default.
- W4367840545 hasPublicationYear "2023" @default.
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