FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4368363405> ?p ?o ?g. }

• W4368363405 abstract "Abstract Background Mesenchymal stromal cells (MSCs) have a dynamic secretome that plays a critical role in tissue repair and regeneration. However, studying the MSC secretome in mixed-culture disease models remains challenging. This study aimed to develop a mutant methionyl-tRNA synthetase-based toolkit (MetRS L274G ) to selectively profile secreted proteins from MSCs in mixed-culture systems and demonstrate its potential for investigating MSC responses to pathological stimulation. Methods We used CRISPR/Cas9 homology-directed repair to stably integrate MetRS L274G into cells, enabling the incorporation of the non-canonical amino acid, azidonorleucine (ANL), and facilitating selective protein isolation using click chemistry. MetRS L274G was integrated into both in H4 cells and induced pluripotent stem cells (iPSCs) for a series of proof-of-concept studies. Following iPSC differentiation into induced-MSCs, we validated their identity and co-cultured MetRS L274G -expressing iMSCs with naïve or lipopolysaccharide- (LPS) treated THP-1 cells. We then profiled the iMSC secretome using antibody arrays. Results Our results showed successful integration of MetRS L274G into targeted cells, allowing specific isolation of proteins from mixed-culture environments. We also demonstrated that the secretome of MetRS L274G -expressing iMSCs can be differentiated from that of THP-1 cells in co-culture, and is altered when co-cultured with LPS-treated THP-1 cells compared to naïve THP-1 cells. Conclusions The MetRS L274G -based toolkit we have generated enables selective profiling of the MSC secretome in mixed-culture disease models. This approach has broad applications for examining not only MSC responses to models of pathological conditions, but any other cell type that can be differentiated from iPSCs. This can potentially reveal novel MSC-mediated repair mechanisms and advancing our understanding of tissue regeneration processes." @default.
• W4368363405 created "2023-05-05" @default.
• W4368363405 creator A5000571586 @default.
• W4368363405 creator A5000693728 @default.
• W4368363405 creator A5008632814 @default.
• W4368363405 creator A5019461908 @default.
• W4368363405 creator A5022481370 @default.
• W4368363405 creator A5037693618 @default.
• W4368363405 creator A5038413093 @default.
• W4368363405 creator A5042955023 @default.
• W4368363405 creator A5047551560 @default.
• W4368363405 creator A5078318000 @default.
• W4368363405 creator A5079298262 @default.
• W4368363405 date "2023-05-03" @default.
• W4368363405 modified "2023-10-08" @default.
• W4368363405 title "A Mutant Methionyl-tRNA Synthetase-Based toolkit to assess induced-Mesenchymal Stromal Cell secretome in mixed-culture disease models" @default.
• W4368363405 cites W1533317424 @default.
• W4368363405 cites W1585985640 @default.
• W4368363405 cites W1848563179 @default.
• W4368363405 cites W1965295915 @default.
• W4368363405 cites W2025371271 @default.
• W4368363405 cites W2029206837 @default.
• W4368363405 cites W2056880409 @default.
• W4368363405 cites W2058180357 @default.
• W4368363405 cites W2062523457 @default.
• W4368363405 cites W2067132969 @default.
• W4368363405 cites W2136563184 @default.
• W4368363405 cites W2146729586 @default.
• W4368363405 cites W2157162286 @default.
• W4368363405 cites W2169256396 @default.
• W4368363405 cites W2169872175 @default.
• W4368363405 cites W2310870949 @default.
• W4368363405 cites W2463564939 @default.
• W4368363405 cites W2516078455 @default.
• W4368363405 cites W2535903842 @default.
• W4368363405 cites W2573080176 @default.
• W4368363405 cites W2751352478 @default.
• W4368363405 cites W2754672693 @default.
• W4368363405 cites W2765371514 @default.
• W4368363405 cites W2765450717 @default.
• W4368363405 cites W2769795302 @default.
• W4368363405 cites W2783701887 @default.
• W4368363405 cites W2801952804 @default.
• W4368363405 cites W2915097208 @default.
• W4368363405 cites W2996769723 @default.
• W4368363405 cites W2998163934 @default.
• W4368363405 cites W3042695952 @default.
• W4368363405 cites W3088956646 @default.
• W4368363405 cites W3135337399 @default.
• W4368363405 cites W3203317568 @default.
• W4368363405 cites W4220692310 @default.
• W4368363405 cites W4281727248 @default.
• W4368363405 cites W4309506674 @default.
• W4368363405 cites W4313312366 @default.
• W4368363405 cites W4313891146 @default.
• W4368363405 doi "https://doi.org/10.21203/rs.3.rs-2838195/v1" @default.
• W4368363405 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37205579" @default.
• W4368363405 hasPublicationYear "2023" @default.
• W4368363405 type Work @default.
• W4368363405 citedByCount "0" @default.
• W4368363405 crossrefType "posted-content" @default.
• W4368363405 hasAuthorship W4368363405A5000571586 @default.
• W4368363405 hasAuthorship W4368363405A5000693728 @default.
• W4368363405 hasAuthorship W4368363405A5008632814 @default.
• W4368363405 hasAuthorship W4368363405A5019461908 @default.
• W4368363405 hasAuthorship W4368363405A5022481370 @default.
• W4368363405 hasAuthorship W4368363405A5037693618 @default.
• W4368363405 hasAuthorship W4368363405A5038413093 @default.
• W4368363405 hasAuthorship W4368363405A5042955023 @default.
• W4368363405 hasAuthorship W4368363405A5047551560 @default.
• W4368363405 hasAuthorship W4368363405A5078318000 @default.
• W4368363405 hasAuthorship W4368363405A5079298262 @default.
• W4368363405 hasBestOaLocation W43683634051 @default.
• W4368363405 hasConcept C104317684 @default.
• W4368363405 hasConcept C153911025 @default.
• W4368363405 hasConcept C16930146 @default.
• W4368363405 hasConcept C198826908 @default.
• W4368363405 hasConcept C502942594 @default.
• W4368363405 hasConcept C54355233 @default.
• W4368363405 hasConcept C70721500 @default.
• W4368363405 hasConcept C81885089 @default.
• W4368363405 hasConcept C86803240 @default.
• W4368363405 hasConcept C95444343 @default.
• W4368363405 hasConcept C98108389 @default.
• W4368363405 hasConceptScore W4368363405C104317684 @default.
• W4368363405 hasConceptScore W4368363405C153911025 @default.
• W4368363405 hasConceptScore W4368363405C16930146 @default.
• W4368363405 hasConceptScore W4368363405C198826908 @default.
• W4368363405 hasConceptScore W4368363405C502942594 @default.
• W4368363405 hasConceptScore W4368363405C54355233 @default.
• W4368363405 hasConceptScore W4368363405C70721500 @default.
• W4368363405 hasConceptScore W4368363405C81885089 @default.
• W4368363405 hasConceptScore W4368363405C86803240 @default.
• W4368363405 hasConceptScore W4368363405C95444343 @default.
• W4368363405 hasConceptScore W4368363405C98108389 @default.
• W4368363405 hasLocation W43683634051 @default.
• W4368363405 hasLocation W43683634052 @default.
• W4368363405 hasOpenAccess W4368363405 @default.
• W4368363405 hasPrimaryLocation W43683634051 @default.
• W4368363405 hasRelatedWork W2062521268 @default.

• next