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- W4368363611 abstract "Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure." @default.
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- W4368363611 date "2023-05-01" @default.
- W4368363611 modified "2023-10-14" @default.
- W4368363611 title "Establishment of bovine trophoblast stem cells" @default.
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- W4368363611 doi "https://doi.org/10.1016/j.celrep.2023.112439" @default.
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