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- W436903827 abstract "Citrus Huanglongbing is a quarantine disease in China.Identifying geographical distribution of Citrus Huanglongbing is of significance to control its spreading.30 diseased Citrus leaf samples including 19 from Ji'an region and 11 from Fuzhou region were collected in October to November of 2008.All the samples were detected for the pathogen of Citrus huanglongbing by using PCR method.A DNA fragment of about 700 bp was amplified from each of the four samples of Ji'an(one from Suichuan County,two from Wan'an County,and one from Taihe County) and the positive control,while no such fragments were produced from any other samples and the negative control.Three PCR products from their corresponding isolates of Shuangqiao 1 in Suichuan,Yaotou 1 in Wan'an and Wentian 4 in Taihe were purified and sequenced,and a DNA sequence of 654 nucleotides was obtained from each of the three PCR products.Homologous comparison revealed that not only are the three sequences identical,but also are 100% homologous to all reported corresponding sequences of Liberobacter asisaticus in China and abroad.However,there is only 79.6% homology between the three sequences and the corresponding sequence of Liberobacter africanus.The results shows that all the three pathogenic isolates belong to Liberobacter asisaticus.The finding that there is a distribution of Liberobacter asisaticus in Suichuan,Wan'an and Taihe counties of Ji'an region needs adequate attention from relevant departments." @default.
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- W436903827 date "2009-01-01" @default.
- W436903827 modified "2023-09-23" @default.
- W436903827 title "Diagnosis of Citrus Huanglongbing in Ji'an and Fuzhou regions by using PCR and nucleotide sequencing methods." @default.
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