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- W4371781138 abstract "Mitochondrial respiration is an essential component of cellular metabolism. It is a process of energy conversion through enzymatically mediated reactions, the energy of taken-up substrates transformed to the ATP production. Seahorse equipment allows to measure oxygen consumption in living cells and estimate key parameters of mitochondrial respiration in real-time mode. Four key mitochondrial respiration parameters could be measured: basal respiration, ATP-production coupled respiration, maximal respiration, and proton leak. This approach demands the application of mitochondrial inhibitors-oligomycin to inhibit ATP synthase, FCCP-to uncouple the inner mitochondrial membrane and allow maximum electron flux through the electron transport chain, rotenone, and antimycin A to inhibit complexes I and III, respectively. This chapter describes two protocols of seahorse measurements performed on iPSC-derived cardiomyocytes and TAZ knock-out C2C12 cell line." @default.
- W4371781138 created "2023-05-07" @default.
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- W4371781138 date "2023-01-01" @default.
- W4371781138 modified "2023-10-14" @default.
- W4371781138 title "Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness" @default.
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- W4371781138 doi "https://doi.org/10.1007/978-1-0716-3052-5_1" @default.
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