Matches in SemOpenAlex for { <https://semopenalex.org/work/W4372205655> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4372205655 abstract "The human dental pulp represents a promising multipotent stem cell reservoir with pre-eminent regenerative competence that can be harvested from an extracted tooth. The neural crest-derived ecto-mesenchymal origin of dental pulp stem cells (DPSCs) bestows a high degree of plasticity that owes to its multifaceted benefits in tissue repair and regeneration. There are various practical ways of harvesting, maintaining, and proliferating adult stem cells being investigated for their use in regenerative medicine. In this work, we demonstrate the establishment of a primary mesenchymal stem cell culture from dental tissue by the explant culture method. The isolated cells were spindle-shaped and adhered to the plastic surface of the culture plate. The phenotypic characterization of these stem cells showed positive expression of the international society of cell therapy (ISCT)-recommended cell surface markers for MSC, such as CD90, CD73, and CD105. Further, negligible expression of hematopoietic (CD45) and endothelial markers (CD34), and less than 2% expression of HLA-DR markers, confirmed the homogeneity and purity of the DPSC cultures. We further illustrated their multipotency based on differentiation to adipogenic, osteogenic, and chondrogenic lineages. We also induced these cells to differentiate into hepatic-like and neuronal-like cells by adding corresponding stimulation media. This optimized protocol will aid in the cultivation of a highly expandable population of mesenchymal stem cells to be utilized in the laboratory or for preclinical studies. Similar protocols can be incorporated into clinical setups for practicing DPSC-based treatments." @default.
- W4372205655 created "2023-05-07" @default.
- W4372205655 creator A5028196906 @default.
- W4372205655 creator A5029171581 @default.
- W4372205655 creator A5049029781 @default.
- W4372205655 creator A5091102625 @default.
- W4372205655 creator A5091285963 @default.
- W4372205655 date "2023-05-05" @default.
- W4372205655 modified "2023-10-16" @default.
- W4372205655 title "Primary Culture of Dental Pulp Stem Cells" @default.
- W4372205655 doi "https://doi.org/10.3791/65223" @default.
- W4372205655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37212583" @default.
- W4372205655 hasPublicationYear "2023" @default.
- W4372205655 type Work @default.
- W4372205655 citedByCount "0" @default.
- W4372205655 crossrefType "journal-article" @default.
- W4372205655 hasAuthorship W4372205655A5028196906 @default.
- W4372205655 hasAuthorship W4372205655A5029171581 @default.
- W4372205655 hasAuthorship W4372205655A5049029781 @default.
- W4372205655 hasAuthorship W4372205655A5091102625 @default.
- W4372205655 hasAuthorship W4372205655A5091285963 @default.
- W4372205655 hasConcept C10205521 @default.
- W4372205655 hasConcept C10854531 @default.
- W4372205655 hasConcept C116716535 @default.
- W4372205655 hasConcept C123012128 @default.
- W4372205655 hasConcept C136302470 @default.
- W4372205655 hasConcept C198826908 @default.
- W4372205655 hasConcept C201750760 @default.
- W4372205655 hasConcept C202751555 @default.
- W4372205655 hasConcept C203014093 @default.
- W4372205655 hasConcept C20875687 @default.
- W4372205655 hasConcept C2777400515 @default.
- W4372205655 hasConcept C2779093498 @default.
- W4372205655 hasConcept C28328180 @default.
- W4372205655 hasConcept C2910776680 @default.
- W4372205655 hasConcept C54355233 @default.
- W4372205655 hasConcept C86803240 @default.
- W4372205655 hasConcept C95444343 @default.
- W4372205655 hasConceptScore W4372205655C10205521 @default.
- W4372205655 hasConceptScore W4372205655C10854531 @default.
- W4372205655 hasConceptScore W4372205655C116716535 @default.
- W4372205655 hasConceptScore W4372205655C123012128 @default.
- W4372205655 hasConceptScore W4372205655C136302470 @default.
- W4372205655 hasConceptScore W4372205655C198826908 @default.
- W4372205655 hasConceptScore W4372205655C201750760 @default.
- W4372205655 hasConceptScore W4372205655C202751555 @default.
- W4372205655 hasConceptScore W4372205655C203014093 @default.
- W4372205655 hasConceptScore W4372205655C20875687 @default.
- W4372205655 hasConceptScore W4372205655C2777400515 @default.
- W4372205655 hasConceptScore W4372205655C2779093498 @default.
- W4372205655 hasConceptScore W4372205655C28328180 @default.
- W4372205655 hasConceptScore W4372205655C2910776680 @default.
- W4372205655 hasConceptScore W4372205655C54355233 @default.
- W4372205655 hasConceptScore W4372205655C86803240 @default.
- W4372205655 hasConceptScore W4372205655C95444343 @default.
- W4372205655 hasIssue "195" @default.
- W4372205655 hasLocation W43722056551 @default.
- W4372205655 hasLocation W43722056552 @default.
- W4372205655 hasOpenAccess W4372205655 @default.
- W4372205655 hasPrimaryLocation W43722056551 @default.
- W4372205655 hasRelatedWork W1916408841 @default.
- W4372205655 hasRelatedWork W1981018261 @default.
- W4372205655 hasRelatedWork W2108702520 @default.
- W4372205655 hasRelatedWork W2265933042 @default.
- W4372205655 hasRelatedWork W2554582697 @default.
- W4372205655 hasRelatedWork W2606557578 @default.
- W4372205655 hasRelatedWork W2732783500 @default.
- W4372205655 hasRelatedWork W2909956080 @default.
- W4372205655 hasRelatedWork W4372205655 @default.
- W4372205655 hasRelatedWork W4372206323 @default.
- W4372205655 isParatext "false" @default.
- W4372205655 isRetracted "false" @default.
- W4372205655 workType "article" @default.