Matches in SemOpenAlex for { <https://semopenalex.org/work/W4372353039> ?p ?o ?g. }
- W4372353039 endingPage "270" @default.
- W4372353039 startingPage "255" @default.
- W4372353039 abstract "As phospholipids of cell membranes, phosphatidylethanolamine (PE) and phosphatidylserine (PS) play crucial roles in glycerophospholipid metabolism. Broadly, some phospholipid biosynthesis enzymes serve as potential fungicide targets. Therefore, revealing the functions and mechanism of PE biosynthesis in plant pathogens would provide potential targets for crop disease control. We performed analyses including phenotypic characterizations, lipidomics, enzyme activity, site-directed mutagenesis, and chemical inhibition assays to study the function of PS decarboxylase-encoding gene MoPSD2 in rice blast fungus Magnaporthe oryzae. The Mopsd2 mutant was defective in development, lipid metabolism, and plant infection. The PS level increased while PE decreased in Mopsd2, consistent with the enzyme activity. Furthermore, chemical doxorubicin inhibited the enzyme activity of MoPsd2 and showed antifungal activity against 10 phytopathogenic fungi including M. oryzae and reduced disease severity of two crop diseases in the field. Three predicted doxorubicin-interacting residues are important for MoPsd2 functions. Our study demonstrates that MoPsd2 is involved in de novo PE biosynthesis and contributes to the development and plant infection of M. oryzae and that doxorubicin shows broad-spectrum antifungal activity as a fungicide candidate. The study also implicates that bacterium Streptomyces peucetius, which biosynthesizes doxorubicin, could be potentially used as an eco-friendly biocontrol agent." @default.
- W4372353039 created "2023-05-07" @default.
- W4372353039 creator A5002862744 @default.
- W4372353039 creator A5003681868 @default.
- W4372353039 creator A5004408112 @default.
- W4372353039 creator A5009121120 @default.
- W4372353039 creator A5024266337 @default.
- W4372353039 creator A5029938413 @default.
- W4372353039 creator A5037346805 @default.
- W4372353039 creator A5051094765 @default.
- W4372353039 creator A5061489306 @default.
- W4372353039 creator A5063806310 @default.
- W4372353039 creator A5066625018 @default.
- W4372353039 creator A5069530324 @default.
- W4372353039 creator A5071062552 @default.
- W4372353039 creator A5071587907 @default.
- W4372353039 creator A5073288750 @default.
- W4372353039 creator A5091074730 @default.
- W4372353039 date "2023-05-06" @default.
- W4372353039 modified "2023-10-02" @default.
- W4372353039 title "Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad‐spectrum antifungal activity" @default.
- W4372353039 cites W1595781572 @default.
- W4372353039 cites W1982838126 @default.
- W4372353039 cites W1983174095 @default.
- W4372353039 cites W1986297143 @default.
- W4372353039 cites W1988984887 @default.
- W4372353039 cites W1997417034 @default.
- W4372353039 cites W2006568893 @default.
- W4372353039 cites W2008884943 @default.
- W4372353039 cites W2025592839 @default.
- W4372353039 cites W2030016913 @default.
- W4372353039 cites W2035455556 @default.
- W4372353039 cites W2035618305 @default.
- W4372353039 cites W2040253535 @default.
- W4372353039 cites W2047195096 @default.
- W4372353039 cites W2053380205 @default.
- W4372353039 cites W2061632266 @default.
- W4372353039 cites W2065681380 @default.
- W4372353039 cites W2073600426 @default.
- W4372353039 cites W2076566953 @default.
- W4372353039 cites W2105111821 @default.
- W4372353039 cites W2107276925 @default.
- W4372353039 cites W2107277218 @default.
- W4372353039 cites W2108931847 @default.
- W4372353039 cites W2110628442 @default.
- W4372353039 cites W2111759380 @default.
- W4372353039 cites W2112727465 @default.
- W4372353039 cites W2113427739 @default.
- W4372353039 cites W2118013428 @default.
- W4372353039 cites W2122549652 @default.
- W4372353039 cites W2127464671 @default.
- W4372353039 cites W2133517031 @default.
- W4372353039 cites W2137037625 @default.
- W4372353039 cites W2156302583 @default.
- W4372353039 cites W2157513547 @default.
- W4372353039 cites W2157878151 @default.
- W4372353039 cites W2163258998 @default.
- W4372353039 cites W2179438025 @default.
- W4372353039 cites W2184043735 @default.
- W4372353039 cites W2221356507 @default.
- W4372353039 cites W2257123939 @default.
- W4372353039 cites W2300552860 @default.
- W4372353039 cites W2328421912 @default.
- W4372353039 cites W2336040088 @default.
- W4372353039 cites W2412206491 @default.
- W4372353039 cites W2594466337 @default.
- W4372353039 cites W2604685034 @default.
- W4372353039 cites W2620923907 @default.
- W4372353039 cites W2729286457 @default.
- W4372353039 cites W2772769731 @default.
- W4372353039 cites W2800126048 @default.
- W4372353039 cites W2802069929 @default.
- W4372353039 cites W2806043847 @default.
- W4372353039 cites W2893539219 @default.
- W4372353039 cites W2947358442 @default.
- W4372353039 cites W2973285273 @default.
- W4372353039 cites W3006070705 @default.
- W4372353039 cites W3011304164 @default.
- W4372353039 cites W3022064470 @default.
- W4372353039 cites W3024471373 @default.
- W4372353039 cites W3027983581 @default.
- W4372353039 cites W3091726990 @default.
- W4372353039 cites W3091863986 @default.
- W4372353039 cites W3094391454 @default.
- W4372353039 cites W3101452385 @default.
- W4372353039 cites W3175782872 @default.
- W4372353039 cites W3203306871 @default.
- W4372353039 cites W3204423702 @default.
- W4372353039 cites W3208068154 @default.
- W4372353039 cites W4211195302 @default.
- W4372353039 cites W4212893735 @default.
- W4372353039 cites W4220824576 @default.
- W4372353039 cites W4226219594 @default.
- W4372353039 cites W4226244148 @default.
- W4372353039 cites W4241534679 @default.
- W4372353039 cites W4288760338 @default.
- W4372353039 cites W4297497355 @default.
- W4372353039 cites W4297995910 @default.