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- W4375852071 abstract "Abstract A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment." @default.
- W4375852071 created "2023-05-10" @default.
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- W4375852071 date "2023-05-08" @default.
- W4375852071 modified "2023-10-15" @default.
- W4375852071 title "Mitomycin C-induced effects on aerobic methanotrophs in a landfill cover soil; implications of a viral shunt?" @default.
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- W4375852071 doi "https://doi.org/10.1093/femsec/fiad047" @default.
- W4375852071 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37156510" @default.
- W4375852071 hasPublicationYear "2023" @default.
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