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• W4375955912 endingPage "2028" @default.
• W4375955912 startingPage "2015" @default.
• W4375955912 abstract "The reconstitution of basic cellular functions in micrometer-sized liposomes has led to a surge of interest in the construction of synthetic cells. Microscopy and flow cytometry are powerful tools for characterizing biological processes in liposomes with fluorescence readouts. However, applying each method separately leads to a compromise between information-rich imaging by microscopy and statistical population analysis by flow cytometry. To address this shortcoming, we here introduce imaging flow cytometry (IFC) for high-throughput, microscopy-based screening of gene-expressing liposomes in laminar flow. We developed a comprehensive pipeline and analysis toolset based on a commercial IFC instrument and software. About 60 thousands of liposome events were collected per run starting from one microliter of the stock liposome solution. Robust population statistics from individual liposome images was performed based on fluorescence and morphological parameters. This allowed us to quantify complex phenotypes covering a wide range of liposomal states that are relevant for building a synthetic cell. The general applicability, current workflow limitations, and future prospects of IFC in synthetic cell research are finally discussed." @default.
• W4375955912 created "2023-05-10" @default.
• W4375955912 creator A5009422718 @default.
• W4375955912 creator A5017469511 @default.
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• W4375955912 date "2023-05-08" @default.
• W4375955912 modified "2023-09-30" @default.
• W4375955912 title "Imaging Flow Cytometry for High-Throughput Phenotyping of Synthetic Cells" @default.
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• W4375955912 doi "https://doi.org/10.1021/acssynbio.3c00074" @default.
• W4375955912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37155828" @default.
• W4375955912 hasPublicationYear "2023" @default.
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