Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376111172> ?p ?o ?g. }
- W4376111172 abstract "Single-stranded DNA gaps form within the E. coli chromosome during replication, repair and recombination. However, information about the extent of ssDNA creation in the genome is limited. To complement a recent whole-genome sequencing study revealing ssDNA gap genomic distribution, size, and frequency, we used fluorescence microscopy to monitor the spatiotemporal dynamics of single-stranded DNA within live E. coli cells. The ssDNA was marked by a functional fluorescent protein fusion of the SSB protein that replaces the wild type SSB. During log-phase growth the SSB fusion produces a mixture of punctate foci and diffuse fluorescence spread throughout the cytosol. Many foci are clustered. Fluorescent markers of DNA polymerase III frequently co-localize with SSB foci, often localizing to the outer edge of the large SSB features. Novel SSB-enriched features form and resolve regularly during normal growth. UV irradiation induces a rapid increase in SSB foci intensity and produces large features composed of multiple partially overlapping foci. The results provide a critical baseline for further exploration of ssDNA generation during DNA metabolism. Alterations in the patterns seen in a mutant lacking RecB function tentatively suggest associations of particular SSB features with the repair of double strand breaks and post-replication gaps." @default.
- W4376111172 created "2023-05-12" @default.
- W4376111172 creator A5009865471 @default.
- W4376111172 creator A5014875441 @default.
- W4376111172 creator A5022463488 @default.
- W4376111172 creator A5052817447 @default.
- W4376111172 creator A5056379010 @default.
- W4376111172 creator A5059504509 @default.
- W4376111172 creator A5062624967 @default.
- W4376111172 creator A5075210236 @default.
- W4376111172 creator A5075591359 @default.
- W4376111172 creator A5080771015 @default.
- W4376111172 date "2023-05-09" @default.
- W4376111172 modified "2023-10-17" @default.
- W4376111172 title "Spatiotemporal Dynamics of Single-stranded DNA Intermediates in<i>Escherichia coli</i>" @default.
- W4376111172 cites W1483134102 @default.
- W4376111172 cites W1501619406 @default.
- W4376111172 cites W1511563273 @default.
- W4376111172 cites W1585911315 @default.
- W4376111172 cites W1652360688 @default.
- W4376111172 cites W1806123628 @default.
- W4376111172 cites W1963782006 @default.
- W4376111172 cites W1971178460 @default.
- W4376111172 cites W1972974900 @default.
- W4376111172 cites W1981829426 @default.
- W4376111172 cites W1989108993 @default.
- W4376111172 cites W1992140093 @default.
- W4376111172 cites W1995692911 @default.
- W4376111172 cites W2006090430 @default.
- W4376111172 cites W2011351273 @default.
- W4376111172 cites W2014571977 @default.
- W4376111172 cites W2017784165 @default.
- W4376111172 cites W2017979067 @default.
- W4376111172 cites W2025007049 @default.
- W4376111172 cites W2030882132 @default.
- W4376111172 cites W2049601212 @default.
- W4376111172 cites W2056467631 @default.
- W4376111172 cites W2066638568 @default.
- W4376111172 cites W2069361954 @default.
- W4376111172 cites W2070742295 @default.
- W4376111172 cites W2075213761 @default.
- W4376111172 cites W2084530022 @default.
- W4376111172 cites W2084802036 @default.
- W4376111172 cites W2093065152 @default.
- W4376111172 cites W2093235875 @default.
- W4376111172 cites W2099412396 @default.
- W4376111172 cites W2116137883 @default.
- W4376111172 cites W2134153749 @default.
- W4376111172 cites W2145532900 @default.
- W4376111172 cites W2147755035 @default.
- W4376111172 cites W2157886738 @default.
- W4376111172 cites W2159438911 @default.
- W4376111172 cites W2165108940 @default.
- W4376111172 cites W2166572577 @default.
- W4376111172 cites W2167279371 @default.
- W4376111172 cites W2178168667 @default.
- W4376111172 cites W2191824721 @default.
- W4376111172 cites W2515298977 @default.
- W4376111172 cites W2578089483 @default.
- W4376111172 cites W2744835540 @default.
- W4376111172 cites W2781499568 @default.
- W4376111172 cites W2784728212 @default.
- W4376111172 cites W2794497310 @default.
- W4376111172 cites W2801044976 @default.
- W4376111172 cites W2907413522 @default.
- W4376111172 cites W2910761535 @default.
- W4376111172 cites W2914168385 @default.
- W4376111172 cites W2957110995 @default.
- W4376111172 cites W2982348926 @default.
- W4376111172 cites W2982587873 @default.
- W4376111172 cites W3011150760 @default.
- W4376111172 cites W3022933121 @default.
- W4376111172 cites W3027665586 @default.
- W4376111172 cites W3044617970 @default.
- W4376111172 cites W3115979224 @default.
- W4376111172 cites W3183081917 @default.
- W4376111172 cites W3198736369 @default.
- W4376111172 cites W4200124957 @default.
- W4376111172 cites W4200267640 @default.
- W4376111172 cites W4232090872 @default.
- W4376111172 cites W4282010062 @default.
- W4376111172 doi "https://doi.org/10.1101/2023.05.08.539320" @default.
- W4376111172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37214928" @default.
- W4376111172 hasPublicationYear "2023" @default.
- W4376111172 type Work @default.
- W4376111172 citedByCount "0" @default.
- W4376111172 crossrefType "posted-content" @default.
- W4376111172 hasAuthorship W4376111172A5009865471 @default.
- W4376111172 hasAuthorship W4376111172A5014875441 @default.
- W4376111172 hasAuthorship W4376111172A5022463488 @default.
- W4376111172 hasAuthorship W4376111172A5052817447 @default.
- W4376111172 hasAuthorship W4376111172A5056379010 @default.
- W4376111172 hasAuthorship W4376111172A5059504509 @default.
- W4376111172 hasAuthorship W4376111172A5062624967 @default.
- W4376111172 hasAuthorship W4376111172A5075210236 @default.
- W4376111172 hasAuthorship W4376111172A5075591359 @default.
- W4376111172 hasAuthorship W4376111172A5080771015 @default.
- W4376111172 hasBestOaLocation W43761111721 @default.
- W4376111172 hasConcept C102744134 @default.
- W4376111172 hasConcept C143425029 @default.