Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376121492> ?p ?o ?g. }
- W4376121492 endingPage "12" @default.
- W4376121492 startingPage "12" @default.
- W4376121492 abstract "The purpose of this study was to determine whether corneal collagen cross-linking (CXL) alters fungal susceptibility and increases the severity of keratitis through macrophage activation in rats.Four weeks following CXL pretreatment, the corneal epithelium of adult rats was removed and inoculated with Candida albicans (C. albicans; CXL + inoculation group). The non-CXL-pretreated corneas were also inoculated with C. albicans (inoculation group). Clinical scoring and histopathological examination were performed to determine the severity of fungal keratitis. Immunofluorescence and confocal microscopy imaging were applied to determine the effects of CXL treatment on corneal local macrophage content. Real-time polymerase chain reaction (RT-PCR) and Western blots were used to evaluate mRNA and protein expression. Flow cytometry assays were performed to detect M1- and M2-type macrophages.CXL pretreatment (CXL + inoculation) resulted in higher infection success rate and more severe fungal keratitis than inoculation alone (inoculation group). On days 1, 3, and 7 following fungal infection, the increase in macrophage infiltration and IL-1β, MMP-9, and VEGFA expression was greater in the CXL + inoculation group than in the inoculation group. Number of M1- and M2-type macrophages, M1 to M2 ratio, M1-type macrophage genes, inducible nitric oxide synthase (iNOS), and tumor necrosis factor (TNFα) expression were higher in the CXL + inoculation group compared with the inoculation group.Our data demonstrate that CXL may increase the colonization of macrophages and activate more M1-type macrophages to increase fungal susceptibility and severity of keratitis.This study may aid long-term risk assessment and treatment of the complications of CXL." @default.
- W4376121492 created "2023-05-12" @default.
- W4376121492 creator A5001030255 @default.
- W4376121492 creator A5004081052 @default.
- W4376121492 creator A5012717507 @default.
- W4376121492 creator A5061511134 @default.
- W4376121492 creator A5062617219 @default.
- W4376121492 creator A5071844040 @default.
- W4376121492 creator A5071963696 @default.
- W4376121492 date "2023-05-10" @default.
- W4376121492 modified "2023-10-18" @default.
- W4376121492 title "Effect of Corneal Collagen Cross-Linking on Subsequent Corneal Fungal Infection in Rats" @default.
- W4376121492 cites W1514239926 @default.
- W4376121492 cites W1528388797 @default.
- W4376121492 cites W1965859404 @default.
- W4376121492 cites W1968347085 @default.
- W4376121492 cites W1970412040 @default.
- W4376121492 cites W1975634954 @default.
- W4376121492 cites W1986938496 @default.
- W4376121492 cites W1995783950 @default.
- W4376121492 cites W2011199583 @default.
- W4376121492 cites W2016350263 @default.
- W4376121492 cites W2027087089 @default.
- W4376121492 cites W2038689279 @default.
- W4376121492 cites W2055232534 @default.
- W4376121492 cites W2065210647 @default.
- W4376121492 cites W2084153517 @default.
- W4376121492 cites W2095100716 @default.
- W4376121492 cites W2100299975 @default.
- W4376121492 cites W2113430923 @default.
- W4376121492 cites W2131227412 @default.
- W4376121492 cites W2170316875 @default.
- W4376121492 cites W2225007036 @default.
- W4376121492 cites W2321997958 @default.
- W4376121492 cites W2557531538 @default.
- W4376121492 cites W2587387014 @default.
- W4376121492 cites W2612879211 @default.
- W4376121492 cites W2639110935 @default.
- W4376121492 cites W2792797980 @default.
- W4376121492 cites W2793011605 @default.
- W4376121492 cites W2883748212 @default.
- W4376121492 cites W2939267438 @default.
- W4376121492 cites W2940209515 @default.
- W4376121492 cites W2971817149 @default.
- W4376121492 cites W2972676927 @default.
- W4376121492 cites W2979580350 @default.
- W4376121492 cites W2985963434 @default.
- W4376121492 cites W3004188243 @default.
- W4376121492 cites W3013522609 @default.
- W4376121492 cites W3035185392 @default.
- W4376121492 cites W3041482320 @default.
- W4376121492 cites W3044874265 @default.
- W4376121492 cites W3124915095 @default.
- W4376121492 cites W3171957076 @default.
- W4376121492 cites W4200163733 @default.
- W4376121492 cites W4211119105 @default.
- W4376121492 cites W4251663120 @default.
- W4376121492 doi "https://doi.org/10.1167/tvst.12.5.12" @default.
- W4376121492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37163284" @default.
- W4376121492 hasPublicationYear "2023" @default.
- W4376121492 type Work @default.
- W4376121492 citedByCount "0" @default.
- W4376121492 crossrefType "journal-article" @default.
- W4376121492 hasAuthorship W4376121492A5001030255 @default.
- W4376121492 hasAuthorship W4376121492A5004081052 @default.
- W4376121492 hasAuthorship W4376121492A5012717507 @default.
- W4376121492 hasAuthorship W4376121492A5061511134 @default.
- W4376121492 hasAuthorship W4376121492A5062617219 @default.
- W4376121492 hasAuthorship W4376121492A5071844040 @default.
- W4376121492 hasAuthorship W4376121492A5071963696 @default.
- W4376121492 hasBestOaLocation W43761214921 @default.
- W4376121492 hasConcept C142724271 @default.
- W4376121492 hasConcept C17991360 @default.
- W4376121492 hasConcept C180032290 @default.
- W4376121492 hasConcept C202751555 @default.
- W4376121492 hasConcept C203014093 @default.
- W4376121492 hasConcept C2776079991 @default.
- W4376121492 hasConcept C2778560704 @default.
- W4376121492 hasConcept C2779244956 @default.
- W4376121492 hasConcept C2780917455 @default.
- W4376121492 hasConcept C2909898526 @default.
- W4376121492 hasConcept C54355233 @default.
- W4376121492 hasConcept C55493867 @default.
- W4376121492 hasConcept C71924100 @default.
- W4376121492 hasConcept C86803240 @default.
- W4376121492 hasConcept C89423630 @default.
- W4376121492 hasConceptScore W4376121492C142724271 @default.
- W4376121492 hasConceptScore W4376121492C17991360 @default.
- W4376121492 hasConceptScore W4376121492C180032290 @default.
- W4376121492 hasConceptScore W4376121492C202751555 @default.
- W4376121492 hasConceptScore W4376121492C203014093 @default.
- W4376121492 hasConceptScore W4376121492C2776079991 @default.
- W4376121492 hasConceptScore W4376121492C2778560704 @default.
- W4376121492 hasConceptScore W4376121492C2779244956 @default.
- W4376121492 hasConceptScore W4376121492C2780917455 @default.
- W4376121492 hasConceptScore W4376121492C2909898526 @default.
- W4376121492 hasConceptScore W4376121492C54355233 @default.
- W4376121492 hasConceptScore W4376121492C55493867 @default.