Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376131325> ?p ?o ?g. }
- W4376131325 endingPage "2186.e5" @default.
- W4376131325 startingPage "2175" @default.
- W4376131325 abstract "Most eukaryotes respire oxygen, using it to generate biomass and energy. However, a few organisms have lost the capacity to respire. Understanding how they manage biomass and energy production may illuminate the critical points at which respiration feeds into central carbon metabolism and explain possible routes to its optimization. Here, we use two related fission yeasts, Schizosaccharomyces pombe and Schizosaccharomyces japonicus, as a comparative model system. We show that although S. japonicus does not respire oxygen, unlike S. pombe, it is capable of efficient NADH oxidation, amino acid synthesis, and ATP generation. We probe possible optimization strategies through the use of stable isotope tracing metabolomics, mass isotopologue distribution analysis, genetics, and physiological experiments. S. japonicus appears to have optimized cytosolic NADH oxidation via glycerol-3-phosphate synthesis. It runs a fully bifurcated TCA pathway, sustaining amino acid production. Finally, we propose that it has optimized glycolysis to maintain high ATP/ADP ratio, in part by using the pentose phosphate pathway as a glycolytic shunt, reducing allosteric inhibition of glycolysis and supporting biomass generation. By comparing two related organisms with vastly different metabolic strategies, our work highlights the versatility and plasticity of central carbon metabolism in eukaryotes, illuminating critical adaptations supporting the preferential use of glycolysis over oxidative phosphorylation." @default.
- W4376131325 created "2023-05-12" @default.
- W4376131325 creator A5010142228 @default.
- W4376131325 creator A5039431331 @default.
- W4376131325 creator A5039682543 @default.
- W4376131325 creator A5072442606 @default.
- W4376131325 creator A5090205783 @default.
- W4376131325 date "2023-06-01" @default.
- W4376131325 modified "2023-10-16" @default.
- W4376131325 title "Optimization of energy production and central carbon metabolism in a non-respiring eukaryote" @default.
- W4376131325 cites W1487583170 @default.
- W4376131325 cites W1508154574 @default.
- W4376131325 cites W1575115305 @default.
- W4376131325 cites W1782885961 @default.
- W4376131325 cites W1867360977 @default.
- W4376131325 cites W1910125601 @default.
- W4376131325 cites W1949786344 @default.
- W4376131325 cites W1968806777 @default.
- W4376131325 cites W1969376872 @default.
- W4376131325 cites W1970070225 @default.
- W4376131325 cites W1985972019 @default.
- W4376131325 cites W1986806259 @default.
- W4376131325 cites W1990265320 @default.
- W4376131325 cites W1995037880 @default.
- W4376131325 cites W1996102432 @default.
- W4376131325 cites W1997076510 @default.
- W4376131325 cites W1999511820 @default.
- W4376131325 cites W2015383894 @default.
- W4376131325 cites W2020232598 @default.
- W4376131325 cites W2022701539 @default.
- W4376131325 cites W2030115946 @default.
- W4376131325 cites W2031440228 @default.
- W4376131325 cites W2031998547 @default.
- W4376131325 cites W2033215577 @default.
- W4376131325 cites W2039220817 @default.
- W4376131325 cites W2042298776 @default.
- W4376131325 cites W2044394667 @default.
- W4376131325 cites W2058929656 @default.
- W4376131325 cites W2061235852 @default.
- W4376131325 cites W2070277174 @default.
- W4376131325 cites W2072742938 @default.
- W4376131325 cites W2073858966 @default.
- W4376131325 cites W2076519973 @default.
- W4376131325 cites W2095150953 @default.
- W4376131325 cites W2099456413 @default.
- W4376131325 cites W2099914203 @default.
- W4376131325 cites W2104340213 @default.
- W4376131325 cites W2104657811 @default.
- W4376131325 cites W2108338825 @default.
- W4376131325 cites W2110638089 @default.
- W4376131325 cites W2113164703 @default.
- W4376131325 cites W2121324014 @default.
- W4376131325 cites W2126941299 @default.
- W4376131325 cites W2130412392 @default.
- W4376131325 cites W2137386173 @default.
- W4376131325 cites W2145944929 @default.
- W4376131325 cites W2147454875 @default.
- W4376131325 cites W2158180844 @default.
- W4376131325 cites W2167279371 @default.
- W4376131325 cites W2167970645 @default.
- W4376131325 cites W2171588190 @default.
- W4376131325 cites W2172296079 @default.
- W4376131325 cites W2194986544 @default.
- W4376131325 cites W2218026231 @default.
- W4376131325 cites W2231133178 @default.
- W4376131325 cites W2280924661 @default.
- W4376131325 cites W2284694651 @default.
- W4376131325 cites W2307026163 @default.
- W4376131325 cites W2338194256 @default.
- W4376131325 cites W2410824598 @default.
- W4376131325 cites W2461939104 @default.
- W4376131325 cites W2550440423 @default.
- W4376131325 cites W2554896117 @default.
- W4376131325 cites W2625708429 @default.
- W4376131325 cites W2733956321 @default.
- W4376131325 cites W2761287607 @default.
- W4376131325 cites W2774450332 @default.
- W4376131325 cites W2782296607 @default.
- W4376131325 cites W2783867527 @default.
- W4376131325 cites W2793924468 @default.
- W4376131325 cites W2796927648 @default.
- W4376131325 cites W2805761162 @default.
- W4376131325 cites W2884709468 @default.
- W4376131325 cites W2907475522 @default.
- W4376131325 cites W2909237760 @default.
- W4376131325 cites W2914004801 @default.
- W4376131325 cites W2950202561 @default.
- W4376131325 cites W2980837812 @default.
- W4376131325 cites W2995257393 @default.
- W4376131325 cites W2999430643 @default.
- W4376131325 cites W3010882806 @default.
- W4376131325 cites W3017345718 @default.
- W4376131325 cites W3025325275 @default.
- W4376131325 cites W3048469064 @default.
- W4376131325 cites W3084357471 @default.
- W4376131325 cites W3113834486 @default.
- W4376131325 cites W3160252872 @default.
- W4376131325 cites W3186269774 @default.