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- W4376131492 abstract "Cryogenic milling, or cryomilling, involves the use of liquid nitrogen to lower the temperature of the biological material and/or the milling process. When applied to the study of subcellular or suborganellar structures and processes, it allows for their rapid extraction from whole cells frozen in the physiological state of choice. This approach has proven to be useful for the study of yeast mitochondrial ribosomes. Following cryomilling of 100 mL of yeast culture, conveniently tagged mitochondrial ribosomes can be immunoprecipitated and purified in native conditions. These ribosomes are suitable for the application of downstream approaches. These include mitoribosome profiling to analyze the mitochondrial translatome or mass spectrometry analyses to assess the mitoribosome proteome in normal growth conditions or under stress, as described in this method." @default.
- W4376131492 created "2023-05-12" @default.
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- W4376131492 date "2023-01-01" @default.
- W4376131492 modified "2023-10-14" @default.
- W4376131492 title "Rapid Cryopurification of the Yeast Mitochondrial Ribosome" @default.
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- W4376131492 doi "https://doi.org/10.1007/978-1-0716-3171-3_9" @default.
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