Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376133029> ?p ?o ?g. }
- W4376133029 endingPage "464" @default.
- W4376133029 startingPage "450" @default.
- W4376133029 abstract "The pluripotency of embryonic stem cells (ESCs) is more accurately viewed as a continuous developmental process rather than a fixed state. However, the factors that play general or state-specific roles in regulating self-renewal in different pluripotency states remain poorly defined. In this study, parallel genome-wide CRISPR/Cas9 knockout (KO) screens were applied in ESCs cultured in the serum plus LIF (SL) and in the 2i plus LIF (2iL) conditions. The candidate genes were classified into seven groups based on their positive or negative effects on self-renewal, and whether this effect was general or state-specific for ESCs under SL and 2iL culture conditions. We characterized the expression and function of genes in these seven groups. The loss of function of novel pluripotent candidate genes Usp28, Zfp598, and Zfp296 was further evaluated in mouse ESCs. Consistent with our screen, the knockout of Usp28 promotes the proliferation of SL-ESCs and 2iL-ESCs, whereas Zfp598 is indispensable for the self-renewal of ESCs under both culture conditions. The cell phenotypes of Zfp296 KO ESCs under SL and 2iL culture conditions were different. Our work provided a valuable resource for dissecting the molecular regulation of ESC self-renewal in different pluripotency states." @default.
- W4376133029 created "2023-05-12" @default.
- W4376133029 creator A5009415930 @default.
- W4376133029 creator A5023454463 @default.
- W4376133029 creator A5029762841 @default.
- W4376133029 creator A5032511920 @default.
- W4376133029 creator A5034855502 @default.
- W4376133029 creator A5034871366 @default.
- W4376133029 creator A5035451903 @default.
- W4376133029 creator A5041664650 @default.
- W4376133029 creator A5043140056 @default.
- W4376133029 creator A5043787630 @default.
- W4376133029 creator A5052715533 @default.
- W4376133029 creator A5055490132 @default.
- W4376133029 creator A5058920358 @default.
- W4376133029 creator A5062572385 @default.
- W4376133029 creator A5068499840 @default.
- W4376133029 creator A5072692179 @default.
- W4376133029 creator A5076027011 @default.
- W4376133029 creator A5077865439 @default.
- W4376133029 creator A5088457426 @default.
- W4376133029 creator A5091733618 @default.
- W4376133029 date "2023-08-01" @default.
- W4376133029 modified "2023-10-17" @default.
- W4376133029 title "Parallel Genome-Wide CRISPR Screens to Identify State-Dependent Self-Renewal Regulators of Mouse Embryonic Stem Cells" @default.
- W4376133029 cites W1925463439 @default.
- W4376133029 cites W1966758700 @default.
- W4376133029 cites W1973260880 @default.
- W4376133029 cites W1974365957 @default.
- W4376133029 cites W2004618496 @default.
- W4376133029 cites W2031211461 @default.
- W4376133029 cites W2031285837 @default.
- W4376133029 cites W2050700594 @default.
- W4376133029 cites W2058521801 @default.
- W4376133029 cites W2064256444 @default.
- W4376133029 cites W2084495183 @default.
- W4376133029 cites W2084521437 @default.
- W4376133029 cites W2123106337 @default.
- W4376133029 cites W2123829995 @default.
- W4376133029 cites W2127749855 @default.
- W4376133029 cites W2130410032 @default.
- W4376133029 cites W2163679188 @default.
- W4376133029 cites W2179438025 @default.
- W4376133029 cites W2201689894 @default.
- W4376133029 cites W2252568502 @default.
- W4376133029 cites W2255144770 @default.
- W4376133029 cites W2334056159 @default.
- W4376133029 cites W2730564452 @default.
- W4376133029 cites W2739283654 @default.
- W4376133029 cites W2789643795 @default.
- W4376133029 cites W2795471900 @default.
- W4376133029 cites W2796523908 @default.
- W4376133029 cites W2836137747 @default.
- W4376133029 cites W2893198166 @default.
- W4376133029 cites W2895153371 @default.
- W4376133029 cites W2900644235 @default.
- W4376133029 cites W2917101543 @default.
- W4376133029 cites W2928665623 @default.
- W4376133029 cites W2942684831 @default.
- W4376133029 cites W2950402574 @default.
- W4376133029 cites W2986764109 @default.
- W4376133029 cites W3014605631 @default.
- W4376133029 cites W3039822709 @default.
- W4376133029 cites W3164721363 @default.
- W4376133029 cites W3179152125 @default.
- W4376133029 cites W3200735940 @default.
- W4376133029 cites W3204403294 @default.
- W4376133029 cites W4205156843 @default.
- W4376133029 cites W4220831425 @default.
- W4376133029 cites W4309506674 @default.
- W4376133029 cites W4311585975 @default.
- W4376133029 doi "https://doi.org/10.1089/scd.2023.0053" @default.
- W4376133029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37166379" @default.
- W4376133029 hasPublicationYear "2023" @default.
- W4376133029 type Work @default.
- W4376133029 citedByCount "0" @default.
- W4376133029 crossrefType "journal-article" @default.
- W4376133029 hasAuthorship W4376133029A5009415930 @default.
- W4376133029 hasAuthorship W4376133029A5023454463 @default.
- W4376133029 hasAuthorship W4376133029A5029762841 @default.
- W4376133029 hasAuthorship W4376133029A5032511920 @default.
- W4376133029 hasAuthorship W4376133029A5034855502 @default.
- W4376133029 hasAuthorship W4376133029A5034871366 @default.
- W4376133029 hasAuthorship W4376133029A5035451903 @default.
- W4376133029 hasAuthorship W4376133029A5041664650 @default.
- W4376133029 hasAuthorship W4376133029A5043140056 @default.
- W4376133029 hasAuthorship W4376133029A5043787630 @default.
- W4376133029 hasAuthorship W4376133029A5052715533 @default.
- W4376133029 hasAuthorship W4376133029A5055490132 @default.
- W4376133029 hasAuthorship W4376133029A5058920358 @default.
- W4376133029 hasAuthorship W4376133029A5062572385 @default.
- W4376133029 hasAuthorship W4376133029A5068499840 @default.
- W4376133029 hasAuthorship W4376133029A5072692179 @default.
- W4376133029 hasAuthorship W4376133029A5076027011 @default.
- W4376133029 hasAuthorship W4376133029A5077865439 @default.
- W4376133029 hasAuthorship W4376133029A5088457426 @default.
- W4376133029 hasAuthorship W4376133029A5091733618 @default.
- W4376133029 hasConcept C104317684 @default.