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- W4376138844 abstract "Within the Svalbard archipelago, Kongsfjorden is an important marine ecosystem that is recognised as one of the main representative Arctic glacial fjords. Prokaryotic organisms are key drivers of important ecological processes such as carbon fluxes, nutrient mineralisation, and energy transfer, as well as sentinels of environmental pollution, especially in sediments, that are a repository of contaminants. In some areas of the Arctic, the structure and metabolic activity of the microbial community in the organic matter turnover and globally in the functioning of the benthic domain are mostly still unknown. A snapshot of the main microbial parameters such as bacterial abundance (by microscopic and plate counts), structure (by 16S rRNA sequencing), and metabolic activity was provided in Ny-Ålesund harbour, contextually in seawater and sediment samples. Fluorogenic substrates were used to assess the microbial ability to utilise organic substrates such as proteins, polysaccharides, and organic phosphates through specific enzymatic assays (leucine aminopeptidase—LAP, beta-glucosidase—ß-GLU, and alkaline phosphatase—AP, respectively). The metabolic profiles of psychrophilic heterotrophic bacterial isolates were also screened using a qualitative assay. The phylogenetic analysis of the microbial community revealed that Proteobacteria prevailed among the observed taxonomic groups. Several of the observed sequences were assigned to clones found in harbours, microbial biofilms, antifouling paints, or oil-polluted facilities of cold environments, highlighting a signature of human pressure on the polar habitat of Ny-Ålesund harbour." @default.
- W4376138844 created "2023-05-12" @default.
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- W4376138844 date "2023-05-10" @default.
- W4376138844 modified "2023-09-30" @default.
- W4376138844 title "A Snapshot of the Taxonomic Composition and Metabolic Activity of the Microbial Community in an Arctic Harbour (Ny-Ålesund, Kongsfjorden, Svalbard)" @default.
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- W4376138844 doi "https://doi.org/10.3390/jmse11051018" @default.
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