Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376224871> ?p ?o ?g. }
- W4376224871 endingPage "767" @default.
- W4376224871 startingPage "752" @default.
- W4376224871 abstract "Borrelia spirochetes are unique among diderm bacteria in their lack of lipopolysaccharide (LPS) in the outer membrane (OM) and their abundance of surface-exposed lipoproteins with major roles in transmission, virulence, and pathogenesis. Despite their importance, little is known about how surface lipoproteins are translocated through the periplasm and the OM. Here, we characterized Borrelia burgdorferi BB0838, a distant homolog of the OM LPS assembly protein LptD. Using a CRISPR interference approach, we showed that BB0838 is required for cell growth and envelope stability. Upon BB0838 knockdown, surface lipoprotein OspA was retained in the inner leaflet of the OM, as determined by its inaccessibility to in situ proteolysis but its presence in OM vesicles. The topology of the OM porin/adhesin P66 remained unaffected. Quantitative mass spectrometry of the B. burgdorferi membrane-associated proteome confirmed the selective periplasmic retention of surface lipoproteins under BB0838 knockdown conditions. Additional analysis identified a single in situ protease-accessible BB0838 peptide that mapped to a predicted β-barrel surface loop. Alphafold Multimer modeled a B. burgdorferi LptB2 FGCAD complex spanning the periplasm. Together, this suggests that BB0838/LptDBb facilitates the essential terminal step in spirochetal surface lipoprotein secretion, using an orthologous OM component of a pathway that secretes LPS in proteobacteria." @default.
- W4376224871 created "2023-05-13" @default.
- W4376224871 creator A5005945896 @default.
- W4376224871 creator A5030628731 @default.
- W4376224871 creator A5078837753 @default.
- W4376224871 creator A5079392980 @default.
- W4376224871 creator A5086228456 @default.
- W4376224871 creator A5086565247 @default.
- W4376224871 date "2023-05-12" @default.
- W4376224871 modified "2023-10-16" @default.
- W4376224871 title "A <i>Borrelia burgdorferi</i> LptD homolog is required for flipping of surface lipoproteins through the spirochetal outer membrane" @default.
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