FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376225691> ?p ?o ?g. }

• W4376225691 endingPage "124642" @default.
• W4376225691 startingPage "124642" @default.
• W4376225691 abstract "Loop-mediated isothermal amplification, or LAMP, is nowadays the most popular isothermal nucleic acid amplification technique. This technique implements a minimum of four primers, named outer (F3/B3) and inner primers (FIP/BIP). The inner primers hybridize in two distinct regions, and some studies have reported that the usage of a linker, typically composed of four thymines, in the middle of these primers can improve assay performance. In addition to this, dual-priming oligonucleotides, DPO, have been reported to provide highly specific reducing non-specific amplifications. Considering the large number of primers implemented in LAMP assays, in the current study the suitability of DPO primers replacing regular outer primers; and their combination with different linker sequences in the inner primers were explored. The results demonstrated that replacing standard F3/B3 by DPO primers does not significantly affect that overall performance of the assay, and provides additional stability to temperature changes. This observations were consistent regardless the type of linker implemented in the inner primers, out of which in the current study a linker composed of thymines significantly outperformed the other options tested, most likely due to a combination of sequence and physical structure." @default.
• W4376225691 created "2023-05-13" @default.
• W4376225691 creator A5002012829 @default.
• W4376225691 creator A5027784217 @default.
• W4376225691 creator A5056752498 @default.
• W4376225691 creator A5057297985 @default.
• W4376225691 creator A5066687709 @default.
• W4376225691 date "2023-08-01" @default.
• W4376225691 modified "2023-09-30" @default.
• W4376225691 title "Evaluation of the effect of outer primer structure, and inner primer linker sequences, in the performance of Loop-mediated isothermal amplification" @default.
• W4376225691 cites W1967899347 @default.
• W4376225691 cites W1978444320 @default.
• W4376225691 cites W1979974453 @default.
• W4376225691 cites W1993878642 @default.
• W4376225691 cites W2007531290 @default.
• W4376225691 cites W2020023709 @default.
• W4376225691 cites W2046612909 @default.
• W4376225691 cites W2069468367 @default.
• W4376225691 cites W2084352418 @default.
• W4376225691 cites W2100877088 @default.
• W4376225691 cites W2105275554 @default.
• W4376225691 cites W2133424486 @default.
• W4376225691 cites W2344732879 @default.
• W4376225691 cites W2463051433 @default.
• W4376225691 cites W2516714919 @default.
• W4376225691 cites W2574635544 @default.
• W4376225691 cites W2590575494 @default.
• W4376225691 cites W2766658376 @default.
• W4376225691 cites W2772617361 @default.
• W4376225691 cites W2792323960 @default.
• W4376225691 cites W2805634366 @default.
• W4376225691 cites W2909805384 @default.
• W4376225691 cites W2944194243 @default.
• W4376225691 cites W2997403528 @default.
• W4376225691 cites W3010993316 @default.
• W4376225691 cites W3013612743 @default.
• W4376225691 cites W3015281969 @default.
• W4376225691 cites W3021813867 @default.
• W4376225691 cites W3041024589 @default.
• W4376225691 cites W3045178645 @default.
• W4376225691 cites W3161714940 @default.
• W4376225691 cites W3184781691 @default.
• W4376225691 cites W4206840134 @default.
• W4376225691 cites W4210765862 @default.
• W4376225691 cites W4296380784 @default.
• W4376225691 cites W4307969355 @default.
• W4376225691 cites W3129951141 @default.
• W4376225691 doi "https://doi.org/10.1016/j.talanta.2023.124642" @default.
• W4376225691 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37167680" @default.
• W4376225691 hasPublicationYear "2023" @default.
• W4376225691 type Work @default.
• W4376225691 citedByCount "0" @default.
• W4376225691 crossrefType "journal-article" @default.
• W4376225691 hasAuthorship W4376225691A5002012829 @default.
• W4376225691 hasAuthorship W4376225691A5027784217 @default.
• W4376225691 hasAuthorship W4376225691A5056752498 @default.
• W4376225691 hasAuthorship W4376225691A5057297985 @default.
• W4376225691 hasAuthorship W4376225691A5066687709 @default.
• W4376225691 hasBestOaLocation W43762256911 @default.
• W4376225691 hasConcept C111919701 @default.
• W4376225691 hasConcept C129312508 @default.
• W4376225691 hasConcept C153911025 @default.
• W4376225691 hasConcept C178790620 @default.
• W4376225691 hasConcept C185592680 @default.
• W4376225691 hasConcept C24107716 @default.
• W4376225691 hasConcept C2777563447 @default.
• W4376225691 hasConcept C2780557392 @default.
• W4376225691 hasConcept C41008148 @default.
• W4376225691 hasConcept C552990157 @default.
• W4376225691 hasConcept C55493867 @default.
• W4376225691 hasConcept C60635243 @default.
• W4376225691 hasConcept C86803240 @default.
• W4376225691 hasConceptScore W4376225691C111919701 @default.
• W4376225691 hasConceptScore W4376225691C129312508 @default.
• W4376225691 hasConceptScore W4376225691C153911025 @default.
• W4376225691 hasConceptScore W4376225691C178790620 @default.
• W4376225691 hasConceptScore W4376225691C185592680 @default.
• W4376225691 hasConceptScore W4376225691C24107716 @default.
• W4376225691 hasConceptScore W4376225691C2777563447 @default.
• W4376225691 hasConceptScore W4376225691C2780557392 @default.
• W4376225691 hasConceptScore W4376225691C41008148 @default.
• W4376225691 hasConceptScore W4376225691C552990157 @default.
• W4376225691 hasConceptScore W4376225691C55493867 @default.
• W4376225691 hasConceptScore W4376225691C60635243 @default.
• W4376225691 hasConceptScore W4376225691C86803240 @default.
• W4376225691 hasLocation W43762256911 @default.
• W4376225691 hasLocation W43762256912 @default.
• W4376225691 hasOpenAccess W4376225691 @default.
• W4376225691 hasPrimaryLocation W43762256911 @default.
• W4376225691 hasRelatedWork W1483317596 @default.
• W4376225691 hasRelatedWork W2043457331 @default.
• W4376225691 hasRelatedWork W2121637148 @default.
• W4376225691 hasRelatedWork W2127206119 @default.
• W4376225691 hasRelatedWork W2323826182 @default.
• W4376225691 hasRelatedWork W2996513751 @default.
• W4376225691 hasRelatedWork W3154985758 @default.
• W4376225691 hasRelatedWork W4205748148 @default.
• W4376225691 hasRelatedWork W4317528890 @default.

• next