Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376272276> ?p ?o ?g. }
- W4376272276 endingPage "108174" @default.
- W4376272276 startingPage "108174" @default.
- W4376272276 abstract "Cardiovascular diseases, such as myocardial infarction, ischemic stroke, and pulmonary embolism, are the most common causes of disability and death worldwide. Blood clot hydrolysis by thrombolytic enzymes and thrombectomy are key clinical interventions. The most widely used thrombolytic enzyme is alteplase, which has been used in clinical practice since 1986. Another clinically used thrombolytic protein is tenecteplase, which has modified epitopes and engineered glycosylation sites, suggesting that carbohydrate modification in thrombolytic enzymes is a viable strategy for their improvement. This comprehensive review summarizes current knowledge on computational and experimental identification of glycosylation sites and glycan identity, together with methods used for their reengineering. Practical examples from previous studies focus on modification of glycosylations in thrombolytics, e.g., alteplase, tenecteplase, reteplase, urokinase, saruplase, and desmoteplase. Collected clinical data on these glycoproteins demonstrate the great potential of this engineering strategy. Outstanding combinatorics originating from multiple glycosylation sites and the vast variety of covalently attached glycan species can be addressed by directed evolution or rational design. Directed evolution pipelines would benefit from more efficient cell-free expression and high-throughput screening assays, while rational design must employ structure prediction by machine learning and in silico characterization by supercomputing. Perspectives on challenges and opportunities for improvement of thrombolytic enzymes by engineering and evolution of protein glycosylation are provided." @default.
- W4376272276 created "2023-05-13" @default.
- W4376272276 creator A5001281717 @default.
- W4376272276 creator A5003693498 @default.
- W4376272276 creator A5006701252 @default.
- W4376272276 creator A5007471774 @default.
- W4376272276 creator A5025198244 @default.
- W4376272276 creator A5032403655 @default.
- W4376272276 creator A5040585952 @default.
- W4376272276 creator A5053716062 @default.
- W4376272276 creator A5065926704 @default.
- W4376272276 creator A5070422569 @default.
- W4376272276 date "2023-09-01" @default.
- W4376272276 modified "2023-10-17" @default.
- W4376272276 title "Identification, characterization, and engineering of glycosylation in thrombolytics" @default.
- W4376272276 cites W1253952512 @default.
- W4376272276 cites W1485147752 @default.
- W4376272276 cites W1497028439 @default.
- W4376272276 cites W1511443069 @default.
- W4376272276 cites W1522947008 @default.
- W4376272276 cites W1527458440 @default.
- W4376272276 cites W1534757774 @default.
- W4376272276 cites W1541028334 @default.
- W4376272276 cites W1548159321 @default.
- W4376272276 cites W1588919588 @default.
- W4376272276 cites W1742160680 @default.
- W4376272276 cites W1824566708 @default.
- W4376272276 cites W1839631757 @default.
- W4376272276 cites W190357091 @default.
- W4376272276 cites W1915519529 @default.
- W4376272276 cites W1954202239 @default.
- W4376272276 cites W1965443132 @default.
- W4376272276 cites W1967051522 @default.
- W4376272276 cites W1967980012 @default.
- W4376272276 cites W1970363110 @default.
- W4376272276 cites W1972184823 @default.
- W4376272276 cites W1972307298 @default.
- W4376272276 cites W1974605172 @default.
- W4376272276 cites W1980227520 @default.
- W4376272276 cites W1984774538 @default.
- W4376272276 cites W1985548346 @default.
- W4376272276 cites W1988070654 @default.
- W4376272276 cites W1990178930 @default.
- W4376272276 cites W1990971755 @default.
- W4376272276 cites W1990987600 @default.
- W4376272276 cites W1992271352 @default.
- W4376272276 cites W1995054314 @default.
- W4376272276 cites W1995217690 @default.
- W4376272276 cites W1997342829 @default.
- W4376272276 cites W1997480931 @default.
- W4376272276 cites W1997984454 @default.
- W4376272276 cites W1998269731 @default.
- W4376272276 cites W1999082606 @default.
- W4376272276 cites W2001821674 @default.
- W4376272276 cites W2003042213 @default.
- W4376272276 cites W2003364720 @default.
- W4376272276 cites W2005261732 @default.
- W4376272276 cites W2005977521 @default.
- W4376272276 cites W2007027037 @default.
- W4376272276 cites W2007578147 @default.
- W4376272276 cites W2009340203 @default.
- W4376272276 cites W2009378206 @default.
- W4376272276 cites W2009417722 @default.
- W4376272276 cites W2010265993 @default.
- W4376272276 cites W2010927211 @default.
- W4376272276 cites W2011027624 @default.
- W4376272276 cites W2011806920 @default.
- W4376272276 cites W2013087285 @default.
- W4376272276 cites W2014281268 @default.
- W4376272276 cites W2014816029 @default.
- W4376272276 cites W2016648636 @default.
- W4376272276 cites W2017355698 @default.
- W4376272276 cites W2017736462 @default.
- W4376272276 cites W2017837864 @default.
- W4376272276 cites W2021437817 @default.
- W4376272276 cites W2021754784 @default.
- W4376272276 cites W2022372977 @default.
- W4376272276 cites W2024747540 @default.
- W4376272276 cites W2024756148 @default.
- W4376272276 cites W2025100761 @default.
- W4376272276 cites W2025754370 @default.
- W4376272276 cites W2029469459 @default.
- W4376272276 cites W2031119708 @default.
- W4376272276 cites W2031922679 @default.
- W4376272276 cites W2031963644 @default.
- W4376272276 cites W2032242846 @default.
- W4376272276 cites W2032989095 @default.
- W4376272276 cites W2033032481 @default.
- W4376272276 cites W2035151503 @default.
- W4376272276 cites W2035444867 @default.
- W4376272276 cites W2035784347 @default.
- W4376272276 cites W2035939690 @default.
- W4376272276 cites W2036140812 @default.
- W4376272276 cites W2036930004 @default.
- W4376272276 cites W2038431234 @default.
- W4376272276 cites W2040035595 @default.
- W4376272276 cites W2040285727 @default.
- W4376272276 cites W2040470885 @default.