FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376274186> ?p ?o ?g. }

• W4376274186 endingPage "3009" @default.
• W4376274186 startingPage "3002" @default.
• W4376274186 abstract "Organisms have developed effective mechanisms to sense the external environment. Human-designed biosensors exploit this natural optimization, where different biological machinery have been adapted to detect the presence of user-defined molecules. Specifically, the pheromone pathway in the model organism Saccharomyces cerevisiae represents a suitable candidate as a synthetic signaling system. Indeed, it expresses just one G-Protein Coupled Receptor (GPCR), Ste2, able to recognize pheromone and initiate the expression of pheromone-dependent genes. To date, the standard procedure to engineer this system relies on the substitution of the yeast GPCR with another one and on the modification of the yeast G-protein to bind the inserted receptor. Here, we propose an innovative computational procedure, based on geometrical and chemical optimization of protein binding pockets, to select the amino acid substitutions required to make the native yeast GPCR able to recognize a user-defined ligand. This procedure would allow the yeast to recognize a wide range of ligands, without a-priori knowledge about a GPCR recognizing them or the corresponding G protein. We used Monte Carlo simulations to design on Ste2 a binding pocket able to recognize epinephrine, selected as a test ligand. We validated Ste2 mutants via molecular docking and molecular dynamics. We verified that the amino acid substitutions we identified make Ste2 able to accommodate and remain firmly bound to epinephrine. Our results indicate that we sampled efficiently the huge space of possible mutants, proposing such a strategy as a promising starting point for the development of a new kind of S.cerevisiae-based biosensors." @default.
• W4376274186 created "2023-05-13" @default.
• W4376274186 creator A5004111266 @default.
• W4376274186 creator A5015294553 @default.
• W4376274186 creator A5035182510 @default.
• W4376274186 creator A5080168003 @default.
• W4376274186 date "2023-01-01" @default.
• W4376274186 modified "2023-09-30" @default.
• W4376274186 title "Computational structural-based GPCR optimization for user-defined ligand: Implications for the development of biosensors" @default.
• W4376274186 cites W1031578623 @default.
• W4376274186 cites W1981956283 @default.
• W4376274186 cites W2003620627 @default.
• W4376274186 cites W2005614924 @default.
• W4376274186 cites W2008649912 @default.
• W4376274186 cites W2021374144 @default.
• W4376274186 cites W2025625972 @default.
• W4376274186 cites W2027978582 @default.
• W4376274186 cites W2028912942 @default.
• W4376274186 cites W2041791898 @default.
• W4376274186 cites W2044573154 @default.
• W4376274186 cites W2057477511 @default.
• W4376274186 cites W2061755747 @default.
• W4376274186 cites W2070741080 @default.
• W4376274186 cites W2090552201 @default.
• W4376274186 cites W2094403981 @default.
• W4376274186 cites W2106648157 @default.
• W4376274186 cites W2121192347 @default.
• W4376274186 cites W2126655531 @default.
• W4376274186 cites W2130479394 @default.
• W4376274186 cites W2134967712 @default.
• W4376274186 cites W2156257459 @default.
• W4376274186 cites W2172251739 @default.
• W4376274186 cites W2177731497 @default.
• W4376274186 cites W2260617737 @default.
• W4376274186 cites W2754403265 @default.
• W4376274186 cites W2776563109 @default.
• W4376274186 cites W2791988510 @default.
• W4376274186 cites W2900437486 @default.
• W4376274186 cites W3021627663 @default.
• W4376274186 cites W3027735529 @default.
• W4376274186 cites W3083464634 @default.
• W4376274186 cites W3088466303 @default.
• W4376274186 cites W3110433276 @default.
• W4376274186 cites W3113235749 @default.
• W4376274186 cites W3114357590 @default.
• W4376274186 cites W3121996554 @default.
• W4376274186 cites W3134631352 @default.
• W4376274186 cites W3163240600 @default.
• W4376274186 cites W3168436232 @default.
• W4376274186 cites W3177828909 @default.
• W4376274186 cites W3181981720 @default.
• W4376274186 cites W3199721927 @default.
• W4376274186 cites W3201822768 @default.
• W4376274186 cites W3204297934 @default.
• W4376274186 cites W3211507591 @default.
• W4376274186 cites W4205107473 @default.
• W4376274186 cites W4221062442 @default.
• W4376274186 cites W4225556449 @default.
• W4376274186 cites W4253661031 @default.
• W4376274186 cites W4283172172 @default.
• W4376274186 cites W4289711843 @default.
• W4376274186 doi "https://doi.org/10.1016/j.csbj.2023.05.004" @default.
• W4376274186 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37249971" @default.
• W4376274186 hasPublicationYear "2023" @default.
• W4376274186 type Work @default.
• W4376274186 citedByCount "0" @default.
• W4376274186 crossrefType "journal-article" @default.
• W4376274186 hasAuthorship W4376274186A5004111266 @default.
• W4376274186 hasAuthorship W4376274186A5015294553 @default.
• W4376274186 hasAuthorship W4376274186A5035182510 @default.
• W4376274186 hasAuthorship W4376274186A5080168003 @default.
• W4376274186 hasBestOaLocation W43762741861 @default.
• W4376274186 hasConcept C104317684 @default.
• W4376274186 hasConcept C116569031 @default.
• W4376274186 hasConcept C135285700 @default.
• W4376274186 hasConcept C143065580 @default.
• W4376274186 hasConcept C159110408 @default.
• W4376274186 hasConcept C170493617 @default.
• W4376274186 hasConcept C185592680 @default.
• W4376274186 hasConcept C2777576037 @default.
• W4376274186 hasConcept C2779222958 @default.
• W4376274186 hasConcept C41685203 @default.
• W4376274186 hasConcept C55493867 @default.
• W4376274186 hasConcept C70721500 @default.
• W4376274186 hasConcept C71924100 @default.
• W4376274186 hasConcept C86803240 @default.
• W4376274186 hasConcept C9418097 @default.
• W4376274186 hasConceptScore W4376274186C104317684 @default.
• W4376274186 hasConceptScore W4376274186C116569031 @default.
• W4376274186 hasConceptScore W4376274186C135285700 @default.
• W4376274186 hasConceptScore W4376274186C143065580 @default.
• W4376274186 hasConceptScore W4376274186C159110408 @default.
• W4376274186 hasConceptScore W4376274186C170493617 @default.
• W4376274186 hasConceptScore W4376274186C185592680 @default.
• W4376274186 hasConceptScore W4376274186C2777576037 @default.
• W4376274186 hasConceptScore W4376274186C2779222958 @default.
• W4376274186 hasConceptScore W4376274186C41685203 @default.
• W4376274186 hasConceptScore W4376274186C55493867 @default.

• next