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- W4376453128 abstract "The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm)." @default.
- W4376453128 created "2023-05-14" @default.
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- W4376453128 date "2017-03-28" @default.
- W4376453128 modified "2023-10-10" @default.
- W4376453128 title "A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione" @default.
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- W4376453128 doi "https://doi.org/10.1002/ange.201702114" @default.
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