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• W4376453487 abstract "No AccessJournal of UrologyInvestigative Urology1 Aug 1997Apoptosis in the Rat Penis After Penile Denervation Lonnie T. Klein, Mark I. Miller, Ralph Buttyan, Anthony J. Raffo, Martin Burchard, Glen Devris, Yi Chen Cao, Carl Olsson, and Ridwan Shabsigh Lonnie T. KleinLonnie T. Klein More articles by this author , Mark I. MillerMark I. Miller More articles by this author , Ralph ButtyanRalph Buttyan More articles by this author , Anthony J. RaffoAnthony J. Raffo More articles by this author , Martin BurchardMartin Burchard More articles by this author , Glen DevrisGlen Devris More articles by this author , Yi Chen CaoYi Chen Cao More articles by this author , Carl OlssonCarl Olsson More articles by this author , and Ridwan ShabsighRidwan Shabsigh More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)64572-5AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Despite the advances in nerve sparing prostatectomy for prostate cancer, some patients develop impotence or subjectively complain of a decrease in penile size. We hypothesized that these clinical observations may be explained by injury to the cavernous nerves resulting in programmed cell death (apoptosis) within the penis. We utilized a rat model of penile denervation in order to demonstrate apoptosis after denervation. Methods and Materials: Fifteen male Sprague Dawley rats underwent abdominal exploration and bilateral cavernous neurotomy. Fifteen sham operations were performed as normal controls. The rats were sacrificed on postoperative day 1, 2, 3, 6, and 10 and their penises were harvested. Messenger RNA was extracted and probed on a northern blot for sulfated glycoprotein-2 (SGP-2). SGP-2 is a gene product reported to be elevated in apoptotic tissues. Separate denervated and sham rats were used for DNA extraction (sacrificed postoperative day #2) in order to demonstrate the internucleosomal DNA fragmentation (laddering) found in apoptotic tissues. In addition, in situ histology was performed with ISEL techniques (in situ end labeling) to stain for apoptotic nuclei in denervated rats. Results: Northern blot analysis showed a large increase in SGP-2 mRNA expression in the denervated rats with little detected in the sham operated group. DNA extraction studies revealed the presence of internucleosomal DNA fragmentation on agarose gel (a marker for apoptosis) in the denervated group versus intact high molecular weight DNA in the sham rats. In addition, in situ staining of denervated penile erectile tissue demonstrated apoptotic nuclei in the cavernous tissue. Conclusion: Apoptosis of penile erectile tissue occurs after denervation of the rat penis. This has not been previously described in the literature and may offer some explanation at the molecular level concerning the mechanism of impotence and/or decrease in penile size after radical prostatectomy. References 1 : Impotence following radical prostatectomy: insight into etiology and prevention.. J. Urol.1982; 128: 492. Abstract, Google Scholar 2 : Sexual function following radical prostatectomy: influence of preservation of neurovascular bundles.. J. Urol.1991; 145: 998. 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Google Scholar Squire Urologic Clinic, Department of Urology, College of Physicians and Surgeons of Columbia University, New York, New York.© 1997 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited ByLysiak J, Yang S, Klausner A, Son H, Tuttle J and Steers W (2018) Tadalafil Increases Akt and Extracellular Signal-Regulated Kinase 1/2 Activation, and Prevents Apoptotic Cell Death in the Penis Following DenervationJournal of Urology, VOL. 179, NO. 2, (779-785), Online publication date: 1-Feb-2008.Mulhall J, Secin F and Guillonneau B (2018) Artery Sparing Radical Prostatectomy—Myth or Reality?Journal of Urology, VOL. 179, NO. 3, (827-831), Online publication date: 1-Mar-2008.Haliloglu A, Baltaci S and Yaman O (2018) Penile Length Changes in Men Treated With Androgen Suppression Plus Radiation Therapy for Local or Locally Advanced Prostate CancerJournal of Urology, VOL. 177, NO. 1, (128-130), Online publication date: 1-Jan-2007.Burnett A and Lue T (2018) Neuromodulatory Therapy to Improve Erectile Function Recovery Outcomes After Pelvic SurgeryJournal of Urology, VOL. 176, NO. 3, (882-887), Online publication date: 1-Sep-2006.USER H, HAIRSTON J, ZELNER D, McKENNA K and McVARY K (2018) Penile Weight and Cell Subtype Specific Changes in a Post-Radical Prostatectomy Model of Erectile DysfunctionJournal of Urology, VOL. 169, NO. 3, (1175-1179), Online publication date: 1-Mar-2003.SAVOIE M, KIM S and SOLOWAY M (2018) A Prospective Study Measuring Penile Length in Men Treated With Radical Prostatectomy for Prostate CancerJournal of Urology, VOL. 169, NO. 4, (1462-1464), Online publication date: 1-Apr-2003.USER H, ZELNER D, McKENNA K and McVARY K (2018) Microarray Analysis and Description of Smr1 Gene in Rat Penis in a Post-Radical Prostatectomy Model of Erectile DysfunctionJournal of Urology, VOL. 170, NO. 1, (298-301), Online publication date: 1-Jul-2003.GONTERO P, FONTANA F, BAGNASACCO A, PANELLA M, KOCJANCIC E, PRETTI G and FREA B (2018) Is There an Optimal Time for Intracavernous Prostaglandin E1 Rehabilitation Following Nonnerve Sparing Radical Prostatectomy? Results From a Hemodynamic Prospective StudyJournal of Urology, VOL. 169, NO. 6, (2166-2169), Online publication date: 1-Jun-2003.YAMANAKA M, SHIRAI M, SHIINA H, SHIRAI M, TANAKA Y, FUJIME M, OKUYAMA A and DAHIYA R (2018) Loss of Anti-apoptotic Genes in Aging Rat CruraJournal of Urology, VOL. 168, NO. 5, (2296-2300), Online publication date: 1-Nov-2002.HALE T, OKABE H, HEATON J and ADAMS M (2018) ANTIHYPERTENSIVE DRUGS INDUCE STRUCTURAL REMODELING OF THE PENILE VASCULATUREJournal of Urology, VOL. 166, NO. 2, (739-745), Online publication date: 1-Aug-2001.KIM E, NATH R, KADMON D, LIPSHULTZ L, MILES B, SLAWIN K, TANG H, WHEELER T and SCARDINO P (2018) BILATERAL NERVE GRAFT DURING RADICAL RETROPUBIC PROSTATECTOMY: 1-YEAR FOLLOWUPJournal of Urology, VOL. 165, NO. 6 Part 1, (1950-1956), Online publication date: 1-Jun-2001.KIM E, SCARDINO P, HAMPEL O, MILLS N, WHEELER T and NATH R (2018) INTERPOSITION OF SURAL NERVE RESTORES FUNCTION OF CAVERNOUS NERVES RESECTED DURING RADICAL PROSTATECTOMYJournal of Urology, VOL. 161, NO. 1, (188-192), Online publication date: 1-Jan-1999. Volume 158Issue 2August 1997Page: 626-630 Advertisement Copyright & Permissions© 1997 by American Urological Association, Inc.MetricsAuthor Information Lonnie T. Klein More articles by this author Mark I. Miller More articles by this author Ralph Buttyan More articles by this author Anthony J. Raffo More articles by this author Martin Burchard More articles by this author Glen Devris More articles by this author Yi Chen Cao More articles by this author Carl Olsson More articles by this author Ridwan Shabsigh More articles by this author Expand All Advertisement PDF DownloadLoading ..." @default.
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• W4376453487 title "Apoptosis in the Rat Penis After Penile Denervation" @default.
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