Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376604080> ?p ?o ?g. }
- W4376604080 abstract "Abstract When striving for maximal throughput at minimal volumes while cultivating close to industrial conditions, simple and robust feeding strategies offer important advantages. Enzyme-mediated glucose cleavage from dextrin is an easy way of imitating continuous fed-batch in the small scale, with no complex equipment required. While the release rate – and thus the feed rate – can be controlled by adapting the enzyme concentration, it strongly depends on the concentration of the involved substances and the environmental conditions. Thus, it is a challenge to use the technology for controlling the specific growth rate, as it is commonly done with feed pumps. For solving this problem, we present here a mathematical model that extends simple Michaelis-Menten kinetics by considering different substrate fractions and can be applied to control the glucose release rate even in high throughput experiments. The fitted model was used during automated microbial cultivations to control the growth rate in quasi-continuous fed-batch processes and to realize different exponential growth rates by intermittent additions of enzyme and dextrin by a liquid handling robot system. We thus present an approach for defined biocatalytically controlled glucose supply of small-scale systems, where – if at all – continuous feeding was only possible with low accuracy or high technical efforts until now." @default.
- W4376604080 created "2023-05-17" @default.
- W4376604080 creator A5001739345 @default.
- W4376604080 creator A5011717841 @default.
- W4376604080 creator A5022278329 @default.
- W4376604080 creator A5039135104 @default.
- W4376604080 creator A5050736523 @default.
- W4376604080 date "2023-05-15" @default.
- W4376604080 modified "2023-10-04" @default.
- W4376604080 title "Modeling of enzyme-mediated glucose release to facilitate continuous feed in miniaturized cultivations" @default.
- W4376604080 cites W1683159282 @default.
- W4376604080 cites W1717366851 @default.
- W4376604080 cites W1977884795 @default.
- W4376604080 cites W1980683435 @default.
- W4376604080 cites W1980740090 @default.
- W4376604080 cites W1989747003 @default.
- W4376604080 cites W2011467657 @default.
- W4376604080 cites W2015725756 @default.
- W4376604080 cites W2018865351 @default.
- W4376604080 cites W2021652954 @default.
- W4376604080 cites W2026970231 @default.
- W4376604080 cites W2027804491 @default.
- W4376604080 cites W2033912870 @default.
- W4376604080 cites W2048891380 @default.
- W4376604080 cites W2057551231 @default.
- W4376604080 cites W2065659861 @default.
- W4376604080 cites W2081036420 @default.
- W4376604080 cites W2083340455 @default.
- W4376604080 cites W2085910615 @default.
- W4376604080 cites W2106430658 @default.
- W4376604080 cites W2139192220 @default.
- W4376604080 cites W2148061469 @default.
- W4376604080 cites W2164306370 @default.
- W4376604080 cites W2170270800 @default.
- W4376604080 cites W2171463471 @default.
- W4376604080 cites W2171685074 @default.
- W4376604080 cites W2177608449 @default.
- W4376604080 cites W2341975311 @default.
- W4376604080 cites W2563492920 @default.
- W4376604080 cites W2886956363 @default.
- W4376604080 cites W2892710893 @default.
- W4376604080 cites W2955844540 @default.
- W4376604080 cites W2961911025 @default.
- W4376604080 cites W2964305843 @default.
- W4376604080 cites W2968359666 @default.
- W4376604080 cites W2970752566 @default.
- W4376604080 cites W3086784814 @default.
- W4376604080 cites W3203412580 @default.
- W4376604080 cites W4200428842 @default.
- W4376604080 cites W4232936184 @default.
- W4376604080 cites W4301163675 @default.
- W4376604080 doi "https://doi.org/10.1101/2023.05.14.540734" @default.
- W4376604080 hasPublicationYear "2023" @default.
- W4376604080 type Work @default.
- W4376604080 citedByCount "0" @default.
- W4376604080 crossrefType "posted-content" @default.
- W4376604080 hasAuthorship W4376604080A5001739345 @default.
- W4376604080 hasAuthorship W4376604080A5011717841 @default.
- W4376604080 hasAuthorship W4376604080A5022278329 @default.
- W4376604080 hasAuthorship W4376604080A5039135104 @default.
- W4376604080 hasAuthorship W4376604080A5050736523 @default.
- W4376604080 hasBestOaLocation W43766040801 @default.
- W4376604080 hasConcept C127413603 @default.
- W4376604080 hasConcept C134306372 @default.
- W4376604080 hasConcept C157764524 @default.
- W4376604080 hasConcept C183696295 @default.
- W4376604080 hasConcept C185592680 @default.
- W4376604080 hasConcept C186060115 @default.
- W4376604080 hasConcept C21880701 @default.
- W4376604080 hasConcept C2778254848 @default.
- W4376604080 hasConcept C33923547 @default.
- W4376604080 hasConcept C41008148 @default.
- W4376604080 hasConcept C529335014 @default.
- W4376604080 hasConcept C55493867 @default.
- W4376604080 hasConcept C555944384 @default.
- W4376604080 hasConcept C75235859 @default.
- W4376604080 hasConcept C76155785 @default.
- W4376604080 hasConcept C86803240 @default.
- W4376604080 hasConceptScore W4376604080C127413603 @default.
- W4376604080 hasConceptScore W4376604080C134306372 @default.
- W4376604080 hasConceptScore W4376604080C157764524 @default.
- W4376604080 hasConceptScore W4376604080C183696295 @default.
- W4376604080 hasConceptScore W4376604080C185592680 @default.
- W4376604080 hasConceptScore W4376604080C186060115 @default.
- W4376604080 hasConceptScore W4376604080C21880701 @default.
- W4376604080 hasConceptScore W4376604080C2778254848 @default.
- W4376604080 hasConceptScore W4376604080C33923547 @default.
- W4376604080 hasConceptScore W4376604080C41008148 @default.
- W4376604080 hasConceptScore W4376604080C529335014 @default.
- W4376604080 hasConceptScore W4376604080C55493867 @default.
- W4376604080 hasConceptScore W4376604080C555944384 @default.
- W4376604080 hasConceptScore W4376604080C75235859 @default.
- W4376604080 hasConceptScore W4376604080C76155785 @default.
- W4376604080 hasConceptScore W4376604080C86803240 @default.
- W4376604080 hasLocation W43766040801 @default.
- W4376604080 hasOpenAccess W4376604080 @default.
- W4376604080 hasPrimaryLocation W43766040801 @default.
- W4376604080 hasRelatedWork W2007718334 @default.
- W4376604080 hasRelatedWork W2089592531 @default.
- W4376604080 hasRelatedWork W2375946931 @default.