FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4376612596> ?p ?o ?g. }

• W4376612596 abstract "Abstract Myosins are important motor proteins that associate with the actin cytoskeleton. Structurally, myosins function as heteromeric complexes where smaller light chains, such as calmodulin (CaM), bind to isoleucine-glutamine (IQ) domains in the neck regions to facilitate mechano-enzymatic activity. We recently identified Arabidopsis CaM-like (CML) proteins, CML13 and CML14 as interactors of proteins containing multiple IQ domains, including a member of the myosin VIII class. Here, using in vivo and in vitro assays we demonstrate that CaM, CML13, and CML14 bind the neck region of all four Arabidopsis myosin VIII isoforms. Among ten CML isoforms tested for in planta binding to myosins VIIIs, CaM, CML13, and CML14 gave the strongest signals using in planta split-luciferase protein-interaction assays. In vitro, recombinant CaM, CML13, and CML14 showed specific, high-affinity, calcium-independent binding to the IQ domains of myosin VIIIs. Subcellular localization analysis indicated that CaM, CML13, and CML14 co-localized to plasma membrane-bound puncta when co-expressed with RFP-myosin fusion proteins containing IQ- and tail-domains of myosin VIIIs. In addition, in vitro actin-motility assays using recombinant myosin holoenzymes demonstrated that CaM, CML13, and CML14 function as light chains for myosin VIIIs. Collectively, our data indicate that Arabidopsis CML13 and CML14 are novel myosin VIII light chains. Highlight Myosins are key proteins in the plant cytoskeleton, but the identity of their light chain components is unknown. Here, we show that calmodulin-like proteins function as novel myosin light chains." @default.
• W4376612596 created "2023-05-17" @default.
• W4376612596 creator A5014341698 @default.
• W4376612596 creator A5021368945 @default.
• W4376612596 creator A5021550952 @default.
• W4376612596 creator A5026824761 @default.
• W4376612596 creator A5028917563 @default.
• W4376612596 creator A5041440670 @default.
• W4376612596 creator A5056389807 @default.
• W4376612596 creator A5062400059 @default.
• W4376612596 creator A5064227673 @default.
• W4376612596 creator A5075938126 @default.
• W4376612596 creator A5087775029 @default.
• W4376612596 date "2023-05-13" @default.
• W4376612596 modified "2023-10-14" @default.
• W4376612596 title "Functional Characterization of Calmodulin-like Proteins, CML13 and CML14, as Novel Light Chains of Arabidopsis Class VIII Myosins" @default.
• W4376612596 cites W1546918094 @default.
• W4376612596 cites W1584689324 @default.
• W4376612596 cites W1725055219 @default.
• W4376612596 cites W1921003950 @default.
• W4376612596 cites W1965661118 @default.
• W4376612596 cites W1967579920 @default.
• W4376612596 cites W1970331854 @default.
• W4376612596 cites W1972489054 @default.
• W4376612596 cites W1978934548 @default.
• W4376612596 cites W1982887195 @default.
• W4376612596 cites W1984991233 @default.
• W4376612596 cites W2013701126 @default.
• W4376612596 cites W2019254387 @default.
• W4376612596 cites W2022566017 @default.
• W4376612596 cites W2030402778 @default.
• W4376612596 cites W2032658351 @default.
• W4376612596 cites W2038071582 @default.
• W4376612596 cites W2044786412 @default.
• W4376612596 cites W2051887883 @default.
• W4376612596 cites W2072622802 @default.
• W4376612596 cites W2073936714 @default.
• W4376612596 cites W2082553691 @default.
• W4376612596 cites W2090922453 @default.
• W4376612596 cites W2095929660 @default.
• W4376612596 cites W2102399361 @default.
• W4376612596 cites W2124241482 @default.
• W4376612596 cites W2126809196 @default.
• W4376612596 cites W2128129897 @default.
• W4376612596 cites W2128777198 @default.
• W4376612596 cites W2130296660 @default.
• W4376612596 cites W2131301912 @default.
• W4376612596 cites W2131955094 @default.
• W4376612596 cites W2135129125 @default.
• W4376612596 cites W2142190485 @default.
• W4376612596 cites W2157153409 @default.
• W4376612596 cites W2158031883 @default.
• W4376612596 cites W2331827685 @default.
• W4376612596 cites W2335508555 @default.
• W4376612596 cites W2346654194 @default.
• W4376612596 cites W2422052409 @default.
• W4376612596 cites W2475939426 @default.
• W4376612596 cites W2521488438 @default.
• W4376612596 cites W2605599071 @default.
• W4376612596 cites W2751181757 @default.
• W4376612596 cites W2763285618 @default.
• W4376612596 cites W2773405305 @default.
• W4376612596 cites W2782190700 @default.
• W4376612596 cites W2788206387 @default.
• W4376612596 cites W2792445539 @default.
• W4376612596 cites W2884631399 @default.
• W4376612596 cites W3091960593 @default.
• W4376612596 cites W3112542480 @default.
• W4376612596 cites W3165067056 @default.
• W4376612596 cites W4205226472 @default.
• W4376612596 cites W4213153506 @default.
• W4376612596 cites W4247413557 @default.
• W4376612596 cites W4255760236 @default.
• W4376612596 cites W4280606287 @default.
• W4376612596 cites W4286512656 @default.
• W4376612596 cites W4308616064 @default.
• W4376612596 cites W4308994593 @default.
• W4376612596 cites W4311126178 @default.
• W4376612596 cites W4323350494 @default.
• W4376612596 cites W4324082959 @default.
• W4376612596 doi "https://doi.org/10.1101/2023.05.12.540561" @default.
• W4376612596 hasPublicationYear "2023" @default.
• W4376612596 type Work @default.
• W4376612596 citedByCount "1" @default.
• W4376612596 countsByYear W43766125962023 @default.
• W4376612596 crossrefType "posted-content" @default.
• W4376612596 hasAuthorship W4376612596A5014341698 @default.
• W4376612596 hasAuthorship W4376612596A5021368945 @default.
• W4376612596 hasAuthorship W4376612596A5021550952 @default.
• W4376612596 hasAuthorship W4376612596A5026824761 @default.
• W4376612596 hasAuthorship W4376612596A5028917563 @default.
• W4376612596 hasAuthorship W4376612596A5041440670 @default.
• W4376612596 hasAuthorship W4376612596A5056389807 @default.
• W4376612596 hasAuthorship W4376612596A5062400059 @default.
• W4376612596 hasAuthorship W4376612596A5064227673 @default.
• W4376612596 hasAuthorship W4376612596A5075938126 @default.
• W4376612596 hasAuthorship W4376612596A5087775029 @default.
• W4376612596 hasBestOaLocation W43766125961 @default.
• W4376612596 hasConcept C104317684 @default.
• W4376612596 hasConcept C123894998 @default.

• next