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- W4376642289 abstract "The metal-resistant bacterium Cupriavidus metallidurans uses its copper resistance components to survive the synergistic toxicity of copper ions and gold complexes in auriferous soils. The cup, cop, cus, and gig determinants encode as central component the Cu(I)-exporting PIB1-type ATPase CupA, the periplasmic Cu(I)-oxidase CopA, the transenvelope efflux system CusCBA, and the Gig system with unknown function, respectively. The interplay of these systems with each other and with glutathione (GSH) was analyzed. Copper resistance in single and multiple mutants up to the quintuple mutant was characterized in dose-response curves, Live/Dead-staining, and atomic copper and glutathione content of the cells. The regulation of the cus and gig determinants was studied using reporter gene fusions and in case of gig also RT-PCR studies, which verified the operon structure of gigPABT. All five systems contributed to copper resistance in the order of importance: Cup, Cop, Cus, GSH, and Gig. Only Cup was able to increase copper resistance of the Δcop Δcup Δcus Δgig ΔgshA quintuple mutant but the other systems were required to increase copper resistance of the Δcop Δcus Δgig ΔgshA quadruple mutant to the parent level. Removal of the Cop system resulted in a clear decrease of copper resistance in most strain backgrounds. Cus cooperated with and partially substituted Cop. Gig and GSH cooperated with Cop, Cus, and Cup. Copper resistance is thus the result of an interplay of many systems. IMPORTANCE The ability of bacteria to maintain homeostasis of the essential-but-toxic Janus-faced element copper is important for their survival in many natural environments but also in case of pathogenic bacteria in their respective host. The most important contributors to copper homeostasis have been identified in the last decades and comprise PIB1-type ATPases, periplasmic copper- and oxygen-dependent copper oxidases, transenvelope efflux systems, and glutathione; however, it is not known how all these players interact. This publication investigates this interplay and describes copper homeostasis as a trait emerging from a network of interacting resistance systems." @default.
- W4376642289 created "2023-05-17" @default.
- W4376642289 creator A5001667216 @default.
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- W4376642289 creator A5033276011 @default.
- W4376642289 creator A5074603425 @default.
- W4376642289 creator A5091954962 @default.
- W4376642289 date "2023-06-28" @default.
- W4376642289 modified "2023-10-01" @default.
- W4376642289 title "Full Copper Resistance in Cupriavidus metallidurans Requires the Interplay of Many Resistance Systems" @default.
- W4376642289 cites W1496817833 @default.
- W4376642289 cites W1528606854 @default.
- W4376642289 cites W1531324256 @default.
- W4376642289 cites W1646495957 @default.
- W4376642289 cites W1731592668 @default.
- W4376642289 cites W1838145843 @default.
- W4376642289 cites W1852894381 @default.
- W4376642289 cites W1909187058 @default.
- W4376642289 cites W1921535438 @default.
- W4376642289 cites W1959259592 @default.
- W4376642289 cites W1965135816 @default.
- W4376642289 cites W1968210490 @default.
- W4376642289 cites W1968740095 @default.
- W4376642289 cites W1969993194 @default.
- W4376642289 cites W1970263231 @default.
- W4376642289 cites W1973014510 @default.
- W4376642289 cites W1974939001 @default.
- W4376642289 cites W1975132689 @default.
- W4376642289 cites W1975541299 @default.
- W4376642289 cites W1978579998 @default.
- W4376642289 cites W1978612964 @default.
- W4376642289 cites W1983010214 @default.
- W4376642289 cites W1983188063 @default.
- W4376642289 cites W1994313247 @default.
- W4376642289 cites W1995732138 @default.
- W4376642289 cites W1996296460 @default.
- W4376642289 cites W1996426369 @default.
- W4376642289 cites W2001392561 @default.
- W4376642289 cites W2004126629 @default.
- W4376642289 cites W2005060189 @default.
- W4376642289 cites W2013364466 @default.
- W4376642289 cites W2019076201 @default.
- W4376642289 cites W2027082920 @default.
- W4376642289 cites W2027229485 @default.
- W4376642289 cites W2027444133 @default.
- W4376642289 cites W2030242240 @default.
- W4376642289 cites W2040061072 @default.
- W4376642289 cites W2040969209 @default.
- W4376642289 cites W204977290 @default.
- W4376642289 cites W2050610995 @default.
- W4376642289 cites W2055594544 @default.
- W4376642289 cites W2064815954 @default.
- W4376642289 cites W2068166540 @default.
- W4376642289 cites W2069289340 @default.
- W4376642289 cites W2069586798 @default.
- W4376642289 cites W2070042354 @default.
- W4376642289 cites W2071921751 @default.
- W4376642289 cites W2077106628 @default.
- W4376642289 cites W2081343196 @default.
- W4376642289 cites W2082931569 @default.
- W4376642289 cites W2083190918 @default.
- W4376642289 cites W2083516192 @default.
- W4376642289 cites W2086948445 @default.
- W4376642289 cites W2088680081 @default.
- W4376642289 cites W2092504141 @default.
- W4376642289 cites W2093238015 @default.
- W4376642289 cites W2094084646 @default.
- W4376642289 cites W2096046927 @default.
- W4376642289 cites W2096063115 @default.
- W4376642289 cites W2096976702 @default.
- W4376642289 cites W2097060692 @default.
- W4376642289 cites W2097951502 @default.
- W4376642289 cites W2103378158 @default.
- W4376642289 cites W2104015113 @default.
- W4376642289 cites W2105716644 @default.
- W4376642289 cites W2105786325 @default.
- W4376642289 cites W2106280952 @default.
- W4376642289 cites W2106758948 @default.
- W4376642289 cites W2108857992 @default.
- W4376642289 cites W2111545176 @default.
- W4376642289 cites W2113755389 @default.
- W4376642289 cites W2113929513 @default.
- W4376642289 cites W2117135041 @default.
- W4376642289 cites W2119099728 @default.
- W4376642289 cites W2120966008 @default.
- W4376642289 cites W2127597865 @default.
- W4376642289 cites W2133067229 @default.
- W4376642289 cites W2135377015 @default.
- W4376642289 cites W2136189294 @default.
- W4376642289 cites W2138139328 @default.
- W4376642289 cites W2139559927 @default.
- W4376642289 cites W2145307718 @default.
- W4376642289 cites W2146749612 @default.
- W4376642289 cites W2147287701 @default.
- W4376642289 cites W2147990023 @default.
- W4376642289 cites W2149176191 @default.
- W4376642289 cites W2153587423 @default.
- W4376642289 cites W2161881748 @default.
- W4376642289 cites W2163649898 @default.