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- W4376646913 abstract "Abstract Single-molecule imaging inside living cells has revealed that transcription factors (TFs) bind to DNA transiently, but a long-standing question is how this transient binding is related to transcription activation. Here, we devised a microscopy method to simultaneously measure transient TF binding at a single locus and the effect of these binding events on transcription. We show that DNA binding of the yeast TF Gal4 activates transcription of a target gene within a few seconds, with at least ∼ 20% efficiency and with a high initiation rate of ∼ 1 RNA/s. Gal4 DNA dissociation decreases transcription rapidly. Moreover, at a gene with multiple binding sites, individual Gal4 molecules only rarely stay bound throughout the entire burst, but instead frequently exchange during a burst to increase the transcriptional burst duration. Our results suggest a mechanism for enhancer regulation in more complex eukaryotes, where TF cooperativity and exchange enable robust transcription activation." @default.
- W4376646913 created "2023-05-17" @default.
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- W4376646913 date "2023-05-15" @default.
- W4376646913 modified "2023-10-16" @default.
- W4376646913 title "Transcription factor exchange enables prolonged transcriptional bursts" @default.
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- W4376646913 doi "https://doi.org/10.1101/2023.05.15.540758" @default.
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