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• W4376647146 abstract "Abstract Ubiquitin widely modifies proteins, thereby regulating most cellular functions. The complexity of ubiquitin signalling necessitates unbiased methods enabling global detection of dynamic protein ubiquitylation. Here, we describe UBIMAX ( UB iquitin target Identification by M ass spectrometry in X enopus egg extracts), which enriches ubiquitin-conjugated proteins and quantifies regulation of protein ubiquitylation under precise and adaptable conditions. We benchmark UBIMAX by investigating DNA double-strand break-responsive ubiquitylation events, identifying previously known targets and revealing the actin-organising protein Dbn1 as a novel major target of DNA damage-induced ubiquitylation. We find that Dbn1 is targeted for proteasomal degradation by the SCF β-Trcp1 ubiquitin ligase, in a conserved mechanism driven by ATM-mediated phosphorylation of a previously uncharacterized β-Trcp1 degron containing an SQ motif. We further show that this degron is sufficient to induce DNA-damage dependent protein degradation of a model substrate. Collectively, we demonstrate UBIMAX’s ability to identify novel targets of stimulus-regulated ubiquitylation and reveal an SCF β-Trcp1 -mediated ubiquitylation mechanism controlled directly by the apical DNA damage response kinases." @default.
• W4376647146 created "2023-05-17" @default.
• W4376647146 creator A5009510298 @default.
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• W4376647146 date "2023-05-15" @default.
• W4376647146 modified "2023-09-30" @default.
• W4376647146 title "Profiling ubiquitin signaling with UBIMAX reveals DNA damage- and SCF^β^TRCP-dependent ubiquitylation of the actin-organizing protein Dbn1" @default.
• W4376647146 cites W142384410 @default.
• W4376647146 cites W1559488195 @default.
• W4376647146 cites W1769736739 @default.
• W4376647146 cites W1832030339 @default.
• W4376647146 cites W1950737868 @default.
• W4376647146 cites W1966034049 @default.
• W4376647146 cites W1972053325 @default.
• W4376647146 cites W1974370722 @default.
• W4376647146 cites W1984828374 @default.
• W4376647146 cites W1986384364 @default.
• W4376647146 cites W1987079136 @default.
• W4376647146 cites W1987710873 @default.
• W4376647146 cites W1994045445 @default.
• W4376647146 cites W1996892694 @default.
• W4376647146 cites W2001450450 @default.
• W4376647146 cites W2003236418 @default.
• W4376647146 cites W2014069479 @default.
• W4376647146 cites W2017619447 @default.
• W4376647146 cites W2020235979 @default.
• W4376647146 cites W2021016692 @default.
• W4376647146 cites W2029330932 @default.
• W4376647146 cites W2031845412 @default.
• W4376647146 cites W2033228162 @default.
• W4376647146 cites W2036806530 @default.
• W4376647146 cites W2039262858 @default.
• W4376647146 cites W2043274355 @default.
• W4376647146 cites W2054370981 @default.
• W4376647146 cites W2061050157 @default.
• W4376647146 cites W2061184183 @default.
• W4376647146 cites W2065362756 @default.
• W4376647146 cites W2067618383 @default.
• W4376647146 cites W2068652435 @default.
• W4376647146 cites W2068784898 @default.
• W4376647146 cites W2075135229 @default.
• W4376647146 cites W2080553628 @default.
• W4376647146 cites W2081122699 @default.
• W4376647146 cites W2085416172 @default.
• W4376647146 cites W2089224506 @default.
• W4376647146 cites W2099945578 @default.
• W4376647146 cites W2101969937 @default.
• W4376647146 cites W2106976977 @default.
• W4376647146 cites W2108654668 @default.
• W4376647146 cites W2119517364 @default.
• W4376647146 cites W2124015317 @default.
• W4376647146 cites W2125079066 @default.
• W4376647146 cites W2126788133 @default.
• W4376647146 cites W2140182533 @default.
• W4376647146 cites W2143538182 @default.
• W4376647146 cites W2149678150 @default.
• W4376647146 cites W2149999139 @default.
• W4376647146 cites W2150379282 @default.
• W4376647146 cites W2150621953 @default.
• W4376647146 cites W2157762194 @default.
• W4376647146 cites W2169630006 @default.
• W4376647146 cites W2170776626 @default.
• W4376647146 cites W2219126548 @default.
• W4376647146 cites W2297808341 @default.
• W4376647146 cites W2396545524 @default.
• W4376647146 cites W2398731353 @default.
• W4376647146 cites W2463195069 @default.
• W4376647146 cites W2525711566 @default.
• W4376647146 cites W2536743529 @default.
• W4376647146 cites W2573767984 @default.
• W4376647146 cites W2578133887 @default.
• W4376647146 cites W2605808634 @default.
• W4376647146 cites W2727471508 @default.
• W4376647146 cites W2765365509 @default.
• W4376647146 cites W2808998679 @default.
• W4376647146 cites W2811227283 @default.
• W4376647146 cites W2908226200 @default.
• W4376647146 cites W2908352025 @default.
• W4376647146 cites W2912076408 @default.
• W4376647146 cites W2916483810 @default.
• W4376647146 cites W3025774990 @default.
• W4376647146 cites W3112378621 @default.
• W4376647146 cites W3150184953 @default.
• W4376647146 cites W3190914074 @default.
• W4376647146 cites W3215022102 @default.
• W4376647146 cites W3217182412 @default.
• W4376647146 cites W4200278223 @default.
• W4376647146 cites W4210757594 @default.
• W4376647146 cites W4291284419 @default.
• W4376647146 doi "https://doi.org/10.1101/2023.05.15.540799" @default.
• W4376647146 hasPublicationYear "2023" @default.
• W4376647146 type Work @default.

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