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• W4376871650 abstract "Abstract N-linked glycosylation is a critical post translational modification of eukaryotic proteins. N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions. Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite. In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS. We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae. Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites. Our data identified 582 N-linked glycoproteins with 1273 N-glycosites. Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins. Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level. These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers." @default.
• W4376871650 created "2023-05-18" @default.
• W4376871650 creator A5001541342 @default.
• W4376871650 creator A5004632098 @default.
• W4376871650 creator A5010851418 @default.
• W4376871650 creator A5038156328 @default.
• W4376871650 creator A5082145317 @default.
• W4376871650 date "2023-05-16" @default.
• W4376871650 modified "2023-09-30" @default.
• W4376871650 title "Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi" @default.
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• W4376871650 doi "https://doi.org/10.1038/s41598-023-34936-9" @default.
• W4376871650 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37193733" @default.
• W4376871650 hasPublicationYear "2023" @default.
• W4376871650 type Work @default.
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• W4376871650 hasAuthorship W4376871650A5001541342 @default.
• W4376871650 hasAuthorship W4376871650A5004632098 @default.
• W4376871650 hasAuthorship W4376871650A5010851418 @default.
• W4376871650 hasAuthorship W4376871650A5038156328 @default.

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