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• W4376875202 abstract "Stiffness plays a central action in plant cell extension. Here, we present a protocol to detect changes in stiffness on the external epidermal cell wall of living plant roots using atomic force microscopy (AFM). We provide generalized instructions for collecting force-distance curves and analysis of stiffness using contact-based mechanical model. With this protocol, and some initial training in AFM, a user is able to perform indentation experiments on 4- and 5-day-old Arabidopsis thaliana and determine stiffness properties. For complete details on the use and execution of this protocol, please refer to Godon et al.1" @default.
• W4376875202 created "2023-05-18" @default.
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• W4376875202 date "2023-06-01" @default.
• W4376875202 modified "2023-10-09" @default.
• W4376875202 title "Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy" @default.
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• W4376875202 doi "https://doi.org/10.1016/j.xpro.2023.102265" @default.
• W4376875202 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37200196" @default.
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