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- W4376876346 abstract "Summary Pseudomonas putida , a soil bacterium widely used for synthetic biology and metabolic engineering, processes glucose through convergent peripheral pathways that ultimately yield 6-phosphogluconate. Such a periplasmic gluconate shunt (PGS), composed by glucose and gluconate dehydrogenases, sequentially transforms glucose into gluconate and 2-ketogluconate. Although the secretion of these organic acids by P . putida has been extensively recognized, the mechanism and spatiotemporal regulation of the PGS remained elusive thus far. To address this challenge, we have developed a novel methodology for metabolic flux analysis, D-fluxomics , based on deuterated sugar substrates. D-Fluxomics demonstrated that the PGS underscores a highly dynamic metabolic architecture in glucose-dependent batch cultures of P . putida , characterized by hierarchical carbon uptake by the PGS throughout the cultivation. Additionally, we show that gluconate and 2-ketogluconate accumulation and consumption can be solely explained as a result of the interplay between growth rate-coupled and decoupled metabolic fluxes. As a consequence, the formation of these acids in the PGS is inversely correlated to the bacterial growth rate—unlike the widely studied overflow metabolism of Escherichia coli and yeast. Our findings, which underline survival strategies of soil bacteria thriving in their natural environments, open new avenues for engineering P . putida towards efficient, sugar-based bioprocesses." @default.
- W4376876346 created "2023-05-18" @default.
- W4376876346 creator A5006911351 @default.
- W4376876346 creator A5013152540 @default.
- W4376876346 creator A5035507317 @default.
- W4376876346 creator A5077522931 @default.
- W4376876346 date "2023-05-16" @default.
- W4376876346 modified "2023-10-17" @default.
- W4376876346 title "Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by<i>Pseudomonas putida</i>" @default.
- W4376876346 cites W1499402002 @default.
- W4376876346 cites W1689703030 @default.
- W4376876346 cites W1838886870 @default.
- W4376876346 cites W1964003757 @default.
- W4376876346 cites W1972918463 @default.
- W4376876346 cites W1982799855 @default.
- W4376876346 cites W1989168955 @default.
- W4376876346 cites W1992019477 @default.
- W4376876346 cites W1998369603 @default.
- W4376876346 cites W2001077777 @default.
- W4376876346 cites W2014662125 @default.
- W4376876346 cites W2014875585 @default.
- W4376876346 cites W2030146871 @default.
- W4376876346 cites W2035602623 @default.
- W4376876346 cites W2035612098 @default.
- W4376876346 cites W2036289648 @default.
- W4376876346 cites W2036583236 @default.
- W4376876346 cites W2042293316 @default.
- W4376876346 cites W2044306109 @default.
- W4376876346 cites W2047195788 @default.
- W4376876346 cites W2048369390 @default.
- W4376876346 cites W2052233004 @default.
- W4376876346 cites W2058701510 @default.
- W4376876346 cites W2067229758 @default.
- W4376876346 cites W2078355959 @default.
- W4376876346 cites W2092504964 @default.
- W4376876346 cites W2097314730 @default.
- W4376876346 cites W2099456413 @default.
- W4376876346 cites W2100639271 @default.
- W4376876346 cites W2118753241 @default.
- W4376876346 cites W2120061656 @default.
- W4376876346 cites W2127436363 @default.
- W4376876346 cites W2129634655 @default.
- W4376876346 cites W2132257939 @default.
- W4376876346 cites W2133786390 @default.
- W4376876346 cites W2139542233 @default.
- W4376876346 cites W2149216252 @default.
- W4376876346 cites W2153975567 @default.
- W4376876346 cites W2155695415 @default.
- W4376876346 cites W2159379185 @default.
- W4376876346 cites W2160418287 @default.
- W4376876346 cites W2166317358 @default.
- W4376876346 cites W2171901564 @default.
- W4376876346 cites W2179324586 @default.
- W4376876346 cites W2276302313 @default.
- W4376876346 cites W2278253772 @default.
- W4376876346 cites W2294781959 @default.
- W4376876346 cites W2345143179 @default.
- W4376876346 cites W2384056196 @default.
- W4376876346 cites W2411832964 @default.
- W4376876346 cites W2416065856 @default.
- W4376876346 cites W2460825526 @default.
- W4376876346 cites W2523354609 @default.
- W4376876346 cites W2744256399 @default.
- W4376876346 cites W2752582497 @default.
- W4376876346 cites W2767088268 @default.
- W4376876346 cites W2778917222 @default.
- W4376876346 cites W2801771445 @default.
- W4376876346 cites W2809386196 @default.
- W4376876346 cites W2887708028 @default.
- W4376876346 cites W2904427689 @default.
- W4376876346 cites W2918663233 @default.
- W4376876346 cites W2921198002 @default.
- W4376876346 cites W2939973938 @default.
- W4376876346 cites W2940001845 @default.
- W4376876346 cites W2947386867 @default.
- W4376876346 cites W2952123522 @default.
- W4376876346 cites W2982500515 @default.
- W4376876346 cites W3003290400 @default.
- W4376876346 cites W3010972153 @default.
- W4376876346 cites W3014845627 @default.
- W4376876346 cites W3041432297 @default.
- W4376876346 cites W3045557083 @default.
- W4376876346 cites W3048958360 @default.
- W4376876346 cites W3110813743 @default.
- W4376876346 cites W3119857353 @default.
- W4376876346 cites W3155577317 @default.
- W4376876346 cites W3156458251 @default.
- W4376876346 cites W3178629730 @default.
- W4376876346 cites W3183840238 @default.
- W4376876346 cites W3206674603 @default.
- W4376876346 cites W4235405440 @default.
- W4376876346 cites W4281743967 @default.
- W4376876346 cites W4281756128 @default.
- W4376876346 cites W4296821946 @default.
- W4376876346 cites W4308192745 @default.
- W4376876346 cites W4367310992 @default.
- W4376876346 cites W55987059 @default.
- W4376876346 doi "https://doi.org/10.1101/2023.05.16.541031" @default.
- W4376876346 hasPublicationYear "2023" @default.
- W4376876346 type Work @default.