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- W4377011076 abstract "Here we describe a novel catalyst-free 1,3-dipolar cycloaddition bioconjugation approach for chemical modification of proteins. The dehydroalanine (Dha)-containing protein reacts with nitrile oxides generated in situ through 1,3-dipolar cycloaddition in fully aqueous-buffered systems. This leads to the formation of a new isoxazoline ring at a pre-defined site (Dha) of the protein. Furthermore, the 1-pyrene isoxazoline-installed annexin V acts as a fluorescent probe, which successfully labels the outer cellular membranes of human cholangiocarcinoma (HuCCA-1) cells for detection of apoptosis." @default.
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- W4377011076 date "2023-07-25" @default.
- W4377011076 modified "2023-09-30" @default.
- W4377011076 title "Protein Modification via Nitrile Oxide−Dehydroalanine Cycloaddition: Formation of Isoxazoline Ring on the Protein Backbone" @default.
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- W4377011076 doi "https://doi.org/10.1002/cbic.202300268" @default.
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