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- W4377011293 abstract "Most biologically relevant and diagnostic mutations in the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genome have been identified in the S gene through global genomic surveillance efforts. However, large-scale whole-genome sequencing (WGS) is still challenging in developing countries due to higher costs, reagent delays and limited infrastructure. Consequently, only a small fraction of SARS-CoV-2 samples are characterized through WGS in these regions. Here, we present a complete workflow consisting of a fast library preparation protocol based on tiled amplification of the S gene, followed by a PCR barcoding step and sequencing using Nanopore platforms. This protocol facilitates fast and cost-effective identification of main variants of concern and mutational surveillance of the S gene. By applying this protocol, report time and overall costs for SARS-CoV-2 variant detection could be reduced, contributing to improved genomic surveillance programmes, particularly in low-income regions." @default.
- W4377011293 created "2023-05-19" @default.
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- W4377011293 date "2023-05-18" @default.
- W4377011293 modified "2023-10-18" @default.
- W4377011293 title "Fast and cost-effective SARS-CoV-2 variant detection using Oxford Nanopore full-length spike gene sequencing" @default.
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- W4377011293 doi "https://doi.org/10.1099/mgen.0.001013" @default.
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