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- W4377011374 abstract "To increase the thermal stability of sucrose isomerase from Erwinia rhapontici NX-5, we designed a comprehensive strategy that combines different thermostabilizing elements. We identified 19 high B value amino acid residues for site-directed mutagenesis. An in silico evaluation of the influence of post-translational modifications on the thermostability was also carried out. The sucrose isomerase variants were expressed in Pichia pastoris X33. Thus, for the first time, we report the expression and characterization of glycosylated sucrose isomerases. The designed mutants K174Q, L202E and K174Q/L202E, showed an increase in their optimal temperature of 5 °C, while their half-lives increased 2.21, 1.73 and 2.89 times, respectively. The mutants showed an increase in activity of 20.3% up to 25.3%. The Km values for the K174Q, L202E, and K174Q/L202E mutants decreased by 5.1%, 7.9%, and 9.4%, respectively; furthermore, the catalytic efficiency increased by up to 16%. With the comprehensive strategy followed, we successfully obtain engineered mutants more suitable for industrial applications than their counterparts: native (this research) and wild-type from E. rhapontici NX-5, without compromising the catalytic activity of the molecule." @default.
- W4377011374 created "2023-05-19" @default.
- W4377011374 creator A5019796976 @default.
- W4377011374 creator A5023987476 @default.
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- W4377011374 creator A5031895446 @default.
- W4377011374 creator A5041926319 @default.
- W4377011374 creator A5073908447 @default.
- W4377011374 creator A5090112844 @default.
- W4377011374 date "2023-05-18" @default.
- W4377011374 modified "2023-10-18" @default.
- W4377011374 title "Thermostability improvement of sucrose isomerase PalI NX-5: a comprehensive strategy" @default.
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