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• W4377011384 abstract "Three bacterial strains, 1AS11 T , 1AS12 and 1AS13, members of the new symbiovar salignae and isolated from root nodules of Acacia saligna grown in Tunisia, were characterized using a polyphasic approach. All three strains were assigned to the Rhizobium leguminosarum complex on the basis of rrs gene analysis. Phylogenetic analysis based on 1734 nucleotides of four concatenated housekeeping genes ( recA , atpD , glnII and gyrB ) showed that the three strains were distinct from known rhizobia species of the R. leguminosarum complex and clustered as a separate clade within this complex. Phylogenomic analysis of 92 up-to-date bacterial core genes confirmed the unique clade. The digital DNA–DNA hybridization and blast -based average nucleotide identity values for the three strains and phylogenetically related Rhizobium species ranged from 35.9 to 60.0% and 87.16 to 94.58 %, which were lower than the 70 and 96% species delineation thresholds, respectively. The G+C contents of the strains were 60.82–60.92 mol% and the major fatty acids (>4 %) were summed feature 8 (57.81 %; C 18 : 1 ω7 c ) and C 18 : 1 ω7 c 11-methyl (13.24%). Strains 1AS11 T , 1AS12 and 1AS13 could also be differentiated from their closest described species ( Rhizobium indicum , Rhizobium laguerreae and Rhizobium changzhiense ) by phenotypic and physiological properties as well as fatty acid content. Based on the phylogenetic, genomic, physiological, genotypic and chemotaxonomic data presented in this study, strains 1AS11 T , 1AS12 and 1AS13 represent a new species within the genus Rhizobium and we propose the name Rhizobium acaciae sp. nov. The type strain is 1AS11 T (=DSM 113913 T =ACCC 62388 T )." @default.
• W4377011384 created "2023-05-19" @default.
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• W4377011384 date "2023-05-18" @default.
• W4377011384 modified "2023-09-30" @default.
• W4377011384 title "Rhizobium acaciae sp. nov., a new nitrogen-fixing symbiovar isolated from root nodules of Acacia saligna in Tunisia" @default.
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• W4377011384 doi "https://doi.org/10.1099/ijsem.0.005900" @default.
• W4377011384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37199717" @default.
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