Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377011409> ?p ?o ?g. }
- W4377011409 abstract "Abstract Adoptive immune therapies based on the transfer of antigen-specific T cells have been used successfully to treat various cancers and viral infections, but improved techniques are needed to identify optimally protective human T cell receptors (TCRs). Here we present a high-throughput approach to the identification of natively paired human TCRα and TCRβ (TCRα:β) genes encoding heterodimeric TCRs that recognize specific peptide antigens bound to major histocompatibility complex molecules (pMHCs). We first captured and cloned TCRα:β genes from individual cells, ensuring fidelity using a suppression PCR. We then screened TCRα:β libraries expressed in an immortalized cell line using peptide-pulsed antigen-presenting cells and sequenced activated clones to identify the cognate TCRs. Our results validated an experimental pipeline that allows large-scale repertoire datasets to be annotated with functional specificity information, facilitating the discovery of therapeutically relevant TCRs." @default.
- W4377011409 created "2023-05-19" @default.
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- W4377011409 date "2023-05-17" @default.
- W4377011409 modified "2023-09-27" @default.
- W4377011409 title "Cell activation-based screening of natively paired human T cell receptor repertoires" @default.
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- W4377011409 doi "https://doi.org/10.1038/s41598-023-31858-4" @default.
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- W4377011409 hasPublicationYear "2023" @default.
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