Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377013314> ?p ?o ?g. }
- W4377013314 abstract "Summary Mapping the intricate spatial relationships between the many different molecules inside a cell is essential to understanding cellular functions in all their complexity. Super-resolution fluorescence microscopy offers the required spatial resolution but struggles to reveal more than four different targets simultaneously. Exchanging labels in subsequent imaging rounds for multiplexed imaging extends this number but is limited by its low throughput. Here we present a novel imaging method for rapid multiplexed super-resolution microscopy of a nearly unlimited number of molecular targets by leveraging fluorogenic labeling in conjunction with Transient Adapter-mediated switching for high-throughput DNA-PAINT (FLASH-PAINT). We demonstrate the cell biological versatility of FLASH-PAINT in mammalian cells in four applications: i) mapping nine proteins in a single mammalian cell, ii) elucidating the functional organization of primary cilia by nine-target imaging, iii) revealing the changes in proximity of twelve different targets in unperturbed and dissociated Golgi stacks and iv) investigating inter-organelle contacts at 3D super-resolution." @default.
- W4377013314 created "2023-05-19" @default.
- W4377013314 creator A5011932661 @default.
- W4377013314 creator A5028729865 @default.
- W4377013314 creator A5033967319 @default.
- W4377013314 creator A5037258583 @default.
- W4377013314 creator A5055594834 @default.
- W4377013314 creator A5059006111 @default.
- W4377013314 creator A5089242062 @default.
- W4377013314 date "2023-05-18" @default.
- W4377013314 modified "2023-09-29" @default.
- W4377013314 title "Unraveling cellular complexity with unlimited multiplexed super-resolution imaging" @default.
- W4377013314 cites W1974484155 @default.
- W4377013314 cites W1992858116 @default.
- W4377013314 cites W1995143570 @default.
- W4377013314 cites W1995311028 @default.
- W4377013314 cites W2017455861 @default.
- W4377013314 cites W2042789810 @default.
- W4377013314 cites W2054455698 @default.
- W4377013314 cites W2056499061 @default.
- W4377013314 cites W2061447135 @default.
- W4377013314 cites W2066521860 @default.
- W4377013314 cites W2079100427 @default.
- W4377013314 cites W2086857229 @default.
- W4377013314 cites W2096973725 @default.
- W4377013314 cites W2098219715 @default.
- W4377013314 cites W2101336703 @default.
- W4377013314 cites W2102632503 @default.
- W4377013314 cites W2106316394 @default.
- W4377013314 cites W2109281145 @default.
- W4377013314 cites W2131999768 @default.
- W4377013314 cites W2133161896 @default.
- W4377013314 cites W2141711186 @default.
- W4377013314 cites W2159282837 @default.
- W4377013314 cites W2161870393 @default.
- W4377013314 cites W2166247822 @default.
- W4377013314 cites W2168816122 @default.
- W4377013314 cites W2338621936 @default.
- W4377013314 cites W2471708342 @default.
- W4377013314 cites W2520858613 @default.
- W4377013314 cites W2554260837 @default.
- W4377013314 cites W2561697517 @default.
- W4377013314 cites W2581502995 @default.
- W4377013314 cites W2592037468 @default.
- W4377013314 cites W2605479282 @default.
- W4377013314 cites W2616524931 @default.
- W4377013314 cites W2617667835 @default.
- W4377013314 cites W2620484568 @default.
- W4377013314 cites W2752919824 @default.
- W4377013314 cites W2771617786 @default.
- W4377013314 cites W2779346725 @default.
- W4377013314 cites W2785264903 @default.
- W4377013314 cites W2789549681 @default.
- W4377013314 cites W2903204105 @default.
- W4377013314 cites W2923300855 @default.
- W4377013314 cites W2940168307 @default.
- W4377013314 cites W2950435835 @default.
- W4377013314 cites W2977380204 @default.
- W4377013314 cites W2997807949 @default.
- W4377013314 cites W3017611842 @default.
- W4377013314 cites W3023701177 @default.
- W4377013314 cites W3037873178 @default.
- W4377013314 cites W3047081808 @default.
- W4377013314 cites W3146395015 @default.
- W4377013314 cites W3160636143 @default.
- W4377013314 cites W3167557279 @default.
- W4377013314 cites W3176444924 @default.
- W4377013314 cites W3215069361 @default.
- W4377013314 cites W3216829460 @default.
- W4377013314 cites W4220694398 @default.
- W4377013314 cites W4225264425 @default.
- W4377013314 cites W4318305981 @default.
- W4377013314 cites W4318306732 @default.
- W4377013314 doi "https://doi.org/10.1101/2023.05.17.541061" @default.
- W4377013314 hasPublicationYear "2023" @default.
- W4377013314 type Work @default.
- W4377013314 citedByCount "0" @default.
- W4377013314 crossrefType "posted-content" @default.
- W4377013314 hasAuthorship W4377013314A5011932661 @default.
- W4377013314 hasAuthorship W4377013314A5028729865 @default.
- W4377013314 hasAuthorship W4377013314A5033967319 @default.
- W4377013314 hasAuthorship W4377013314A5037258583 @default.
- W4377013314 hasAuthorship W4377013314A5055594834 @default.
- W4377013314 hasAuthorship W4377013314A5059006111 @default.
- W4377013314 hasAuthorship W4377013314A5089242062 @default.
- W4377013314 hasBestOaLocation W43770133141 @default.
- W4377013314 hasConcept C105780059 @default.
- W4377013314 hasConcept C115961682 @default.
- W4377013314 hasConcept C120665830 @default.
- W4377013314 hasConcept C121332964 @default.
- W4377013314 hasConcept C12554922 @default.
- W4377013314 hasConcept C138268822 @default.
- W4377013314 hasConcept C141239990 @default.
- W4377013314 hasConcept C147080431 @default.
- W4377013314 hasConcept C154945302 @default.
- W4377013314 hasConcept C166936260 @default.
- W4377013314 hasConcept C169274487 @default.
- W4377013314 hasConcept C171250308 @default.
- W4377013314 hasConcept C192562407 @default.
- W4377013314 hasConcept C19275194 @default.