FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377013344> ?p ?o ?g. }

• W4377013344 endingPage "93" @default.
• W4377013344 startingPage "92" @default.
• W4377013344 abstract "PURPOSE: The initial stages of lymphedema are characterized by accumulation of interstitial fluid in the tissues. However, the functional effects of interstitial fluid accumulation on surrounding tissue lymphatics remain unknown. We therefore assessed the molecular effects of interstitial fluid collected from the lymphedematous tissues of patients with secondary lymphedema on cultured lymphatic endothelial cells (LECs). METHODS: Extravasated lymph fluid from 4 patients with secondary lymphedema was aspirated and collected at the time of operative debulking of the affected limbs. Primary human dermal lymphatic endothelial cells (HDLECs) were treated in vitro with media supplemented with 15% volume of patient-derived lymph fluid for 12-72h (experimental); controls were treated with media alone. To assess tube forming capacity, HDLECs were seeded onto Matrigel basement membrane for 12-24h to assess tube forming capacity in response to treatment. Following 72h treatment with lymphatic fluid, HDLECs were harvested to assess changes in the expression of lymphatic growth regulators and modulators of vascular permeability via flow cytometry, western blot, and immunohistochemistry (IHC) analysis RESULTS: Using western blot analysis, we found reduced expression of VEGFR3 in HDLECs treated with lymph from 3/4 patients and no changes in overall VEGFR2 quantities in 4/4 experimental groups. Interestingly, treated HDLECs simultaneously exhibited a significant upregulation of cell surface VEGFR3 localization, reduced membrane Neuropilin-2 expression, and no significant changes in amounts of membrane VEGFR2 (p=0.03, p=0.001, p=0.09 respectively). These findings suggest that post translational modifications of VEGFR3 such as alterations in receptor trafficking or internalization may be induced by exposure to lymphatic fluid. Consistent with increased cell surface expression of VEGFR3, we also noted increased AKT, ERK, and eNOS phosphorylation in cells stimulated with lymphatic fluid. However, cells cultured in lymphatic fluid did not exhibit significant differences in tubule formation or cellular proliferation. LECs exposed to lymphatic fluid demonstrated a marked increase in the expression of phospho-VE-Cadherin suggesting that exposure to lymphatic fluid increases permeability of HDLECs. CONCLUSION: Exposure of HDLEC to lymphedema fluid in vitro promotes alterations in the expression and cellular localization of VEGFR3, thereby influencing the activation of downstream signaling pathways. Additionally, lymphedema fluid induces increased vascular permeability in HDLECs by disrupting the function of adherens and tight junction proteins. These findings provide a cellular mechanism by which lymphatic fluid accumulation in the skin can cause lymphatic dysfunction in the early stages of lymphedema." @default.
• W4377013344 created "2023-05-19" @default.
• W4377013344 creator A5014374310 @default.
• W4377013344 creator A5020019910 @default.
• W4377013344 creator A5032341673 @default.
• W4377013344 creator A5041807409 @default.
• W4377013344 creator A5049569084 @default.
• W4377013344 creator A5060660071 @default.
• W4377013344 creator A5071875260 @default.
• W4377013344 creator A5081956660 @default.
• W4377013344 date "2023-05-01" @default.
• W4377013344 modified "2023-10-14" @default.
• W4377013344 title "148. Extravasated Lymph from Secondary Lymphedema Patients Increases Cellular Permeability and Decreases Lymphatic Endothelial Cell VEGFR3 Expression" @default.
• W4377013344 doi "https://doi.org/10.1097/01.gox.0000938176.12923.10" @default.
• W4377013344 hasPublicationYear "2023" @default.
• W4377013344 type Work @default.
• W4377013344 citedByCount "0" @default.
• W4377013344 crossrefType "journal-article" @default.
• W4377013344 hasAuthorship W4377013344A5014374310 @default.
• W4377013344 hasAuthorship W4377013344A5020019910 @default.
• W4377013344 hasAuthorship W4377013344A5032341673 @default.
• W4377013344 hasAuthorship W4377013344A5041807409 @default.
• W4377013344 hasAuthorship W4377013344A5049569084 @default.
• W4377013344 hasAuthorship W4377013344A5060660071 @default.
• W4377013344 hasAuthorship W4377013344A5071875260 @default.
• W4377013344 hasAuthorship W4377013344A5081956660 @default.
• W4377013344 hasBestOaLocation W43770133441 @default.
• W4377013344 hasConcept C104317684 @default.
• W4377013344 hasConcept C121608353 @default.
• W4377013344 hasConcept C126322002 @default.
• W4377013344 hasConcept C142724271 @default.
• W4377013344 hasConcept C181152851 @default.
• W4377013344 hasConcept C185592680 @default.
• W4377013344 hasConcept C2776193487 @default.
• W4377013344 hasConcept C2776415932 @default.
• W4377013344 hasConcept C2776636583 @default.
• W4377013344 hasConcept C2778608917 @default.
• W4377013344 hasConcept C2779013556 @default.
• W4377013344 hasConcept C2779720271 @default.
• W4377013344 hasConcept C2779822359 @default.
• W4377013344 hasConcept C2780394083 @default.
• W4377013344 hasConcept C2780965494 @default.
• W4377013344 hasConcept C2781392513 @default.
• W4377013344 hasConcept C2910366181 @default.
• W4377013344 hasConcept C502942594 @default.
• W4377013344 hasConcept C530470458 @default.
• W4377013344 hasConcept C55493867 @default.
• W4377013344 hasConcept C71924100 @default.
• W4377013344 hasConceptScore W4377013344C104317684 @default.
• W4377013344 hasConceptScore W4377013344C121608353 @default.
• W4377013344 hasConceptScore W4377013344C126322002 @default.
• W4377013344 hasConceptScore W4377013344C142724271 @default.
• W4377013344 hasConceptScore W4377013344C181152851 @default.
• W4377013344 hasConceptScore W4377013344C185592680 @default.
• W4377013344 hasConceptScore W4377013344C2776193487 @default.
• W4377013344 hasConceptScore W4377013344C2776415932 @default.
• W4377013344 hasConceptScore W4377013344C2776636583 @default.
• W4377013344 hasConceptScore W4377013344C2778608917 @default.
• W4377013344 hasConceptScore W4377013344C2779013556 @default.
• W4377013344 hasConceptScore W4377013344C2779720271 @default.
• W4377013344 hasConceptScore W4377013344C2779822359 @default.
• W4377013344 hasConceptScore W4377013344C2780394083 @default.
• W4377013344 hasConceptScore W4377013344C2780965494 @default.
• W4377013344 hasConceptScore W4377013344C2781392513 @default.
• W4377013344 hasConceptScore W4377013344C2910366181 @default.
• W4377013344 hasConceptScore W4377013344C502942594 @default.
• W4377013344 hasConceptScore W4377013344C530470458 @default.
• W4377013344 hasConceptScore W4377013344C55493867 @default.
• W4377013344 hasConceptScore W4377013344C71924100 @default.
• W4377013344 hasIssue "5S" @default.
• W4377013344 hasLocation W43770133441 @default.
• W4377013344 hasLocation W43770133442 @default.
• W4377013344 hasLocation W43770133443 @default.
• W4377013344 hasOpenAccess W4377013344 @default.
• W4377013344 hasPrimaryLocation W43770133441 @default.
• W4377013344 hasRelatedWork W1534482507 @default.
• W4377013344 hasRelatedWork W1990464709 @default.
• W4377013344 hasRelatedWork W2001978403 @default.
• W4377013344 hasRelatedWork W2115374812 @default.
• W4377013344 hasRelatedWork W2159331913 @default.
• W4377013344 hasRelatedWork W2758059005 @default.
• W4377013344 hasRelatedWork W4361242121 @default.
• W4377013344 hasRelatedWork W4361261109 @default.
• W4377013344 hasRelatedWork W4366181647 @default.
• W4377013344 hasRelatedWork W4377013344 @default.
• W4377013344 hasVolume "11" @default.
• W4377013344 isParatext "false" @default.
• W4377013344 isRetracted "false" @default.
• W4377013344 workType "article" @default.