FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4377014068> ?p ?o ?g. }

• W4377014068 abstract "Sesame (Sesamum indicum L.) is an oilseed crop that present agronomic advantages and nutritional contributions in regions where water and soil fertility are limiting. In September 2020 and October 2022, anthracnose symptoms were observed on sesame fields in Mocorito (25°29'04N;107°55'03W) and Guasave (25°45'40N;108°48'44W), Sinaloa, Mexico. The disease incidence was estimated at up to 35 % (10 has) in five fields. Twenty samples were collected with symptoms on the leaves. On leaves, lesions were irregular and necrotic. Colletotrichum-like colonies were consistently isolated on PDA medium and five monoconidial isolates were obtained. One isolate was selected as a representative for morphological characterization, multilocus phylogenetic analysis, and pathogenicity tests. The isolate was deposited in the Culture Collection of Phytopathogenic Fungi of the Biotic Product Development Center at the National Polytechnic Institute under the accession number IPN 13.0101. On PDA, colonies were flat with an entire margin, initially white, then dark gray with black acervuli and setae. The growth rate was 9.3 mm/day. Conidia (n=100) on PDA were hyaloamerosporae, 17.5- 22.7 × 3.6-4.5 μm, smooth-walled, falcated and pointed at both ends, with granular content. Acervuli showed setae acicular (2-3 septate setae) tapered to the apex. The mycelial appressoria were brown, obclavate and irregular. Morphological features matched those of the Colletotrichum truncatum species complex (Damm et al. 2009). For molecular identification, total DNA was extracted, and the internal transcribed spacer (ITS) region (White et al. 1990), and partial sequences of actin (ACT), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were amplified by PCR (Weir et al. 2012) and sequenced. The sequences were deposited in GenBank under accession nos. OQ214919 (ITS), OQ230773 (ACT), and OQ230774 (GAPDH). BLASTn searches in GenBank showed 100%, 100%, and 100% identity to MN842788 (ITS), MG198003 (ACT), and MF682518 (GAPDH) of C. truncatum, respectively. A phylogenetic tree based on the Maximum Likelihood method and Bayesian Inference including published ITS, ACT, and GAPDH sequence data for C. truncatum species complex was generated (Talhinhas and Baroncelli 2021). In the phylogenetic tree, the isolate IPN 13.0101 was placed in the same clade of C. truncatum. Pathogenicity of the isolate IPN 13.0101 was verified on 15 sesame seedlings leaves (Dormilon variety) (15-day-old) disinfected with sodium hypochlorite and sterile water. Each leave was inoculated with 200 µL of a conidial suspension (1 × 106 spores/mL). Five plants non inoculated served as controls. All plants were kept in a moist chamber for two days, and subsequently transferred to a shade house where the temperature ranged from 25 to 30°C. All inoculated leaves developed irregular and necrotic lesions ten days after inoculation, whereas no symptoms were observed on the control leaves. The fungus was consistently re-isolated from the diseased leaves, fulfilling Koch´s postulates. The experiment was conducted twice with similar results. Colletotrichum spp. has been previously reported (Farr and Rossman, 2023) to cause sesame anthracnose in Mexico (Alvarez, 1976), Thailand (Giatgong, 1980) and Cuba (Arnold, 1986), but this is the first report of C. truncatum causing sesame anthracnose in Mexico. This disease is a recurrent problem in sesame fields in Sinaloa, therefore further studies are required to understand its impact." @default.
• W4377014068 created "2023-05-19" @default.
• W4377014068 creator A5015678610 @default.
• W4377014068 creator A5023177470 @default.
• W4377014068 creator A5042654787 @default.
• W4377014068 creator A5080390567 @default.
• W4377014068 creator A5085093260 @default.
• W4377014068 date "2023-10-05" @default.
• W4377014068 modified "2023-10-16" @default.
• W4377014068 title "Occurrence of <i>Colletotrichum truncatum</i> Causing Foliar Spot on Sesame (<i>Sesamum indicum</i>) in Mexico" @default.
• W4377014068 cites W2036839190 @default.
• W4377014068 cites W2120644786 @default.
• W4377014068 cites W3203662871 @default.
• W4377014068 doi "https://doi.org/10.1094/pdis-03-23-0451-pdn" @default.
• W4377014068 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37202216" @default.
• W4377014068 hasPublicationYear "2023" @default.
• W4377014068 type Work @default.
• W4377014068 citedByCount "0" @default.
• W4377014068 crossrefType "journal-article" @default.
• W4377014068 hasAuthorship W4377014068A5015678610 @default.
• W4377014068 hasAuthorship W4377014068A5023177470 @default.
• W4377014068 hasAuthorship W4377014068A5042654787 @default.
• W4377014068 hasAuthorship W4377014068A5080390567 @default.
• W4377014068 hasAuthorship W4377014068A5085093260 @default.
• W4377014068 hasConcept C104317684 @default.
• W4377014068 hasConcept C133479454 @default.
• W4377014068 hasConcept C144027150 @default.
• W4377014068 hasConcept C155794727 @default.
• W4377014068 hasConcept C157369684 @default.
• W4377014068 hasConcept C158710501 @default.
• W4377014068 hasConcept C193252679 @default.
• W4377014068 hasConcept C2779542283 @default.
• W4377014068 hasConcept C2780034373 @default.
• W4377014068 hasConcept C2780928516 @default.
• W4377014068 hasConcept C43143990 @default.
• W4377014068 hasConcept C55493867 @default.
• W4377014068 hasConcept C59822182 @default.
• W4377014068 hasConcept C86803240 @default.
• W4377014068 hasConceptScore W4377014068C104317684 @default.
• W4377014068 hasConceptScore W4377014068C133479454 @default.
• W4377014068 hasConceptScore W4377014068C144027150 @default.
• W4377014068 hasConceptScore W4377014068C155794727 @default.
• W4377014068 hasConceptScore W4377014068C157369684 @default.
• W4377014068 hasConceptScore W4377014068C158710501 @default.
• W4377014068 hasConceptScore W4377014068C193252679 @default.
• W4377014068 hasConceptScore W4377014068C2779542283 @default.
• W4377014068 hasConceptScore W4377014068C2780034373 @default.
• W4377014068 hasConceptScore W4377014068C2780928516 @default.
• W4377014068 hasConceptScore W4377014068C43143990 @default.
• W4377014068 hasConceptScore W4377014068C55493867 @default.
• W4377014068 hasConceptScore W4377014068C59822182 @default.
• W4377014068 hasConceptScore W4377014068C86803240 @default.
• W4377014068 hasLocation W43770140681 @default.
• W4377014068 hasLocation W43770140682 @default.
• W4377014068 hasOpenAccess W4377014068 @default.
• W4377014068 hasPrimaryLocation W43770140681 @default.
• W4377014068 hasRelatedWork W1194853477 @default.
• W4377014068 hasRelatedWork W2011073027 @default.
• W4377014068 hasRelatedWork W2036841229 @default.
• W4377014068 hasRelatedWork W2050965538 @default.
• W4377014068 hasRelatedWork W2346206322 @default.
• W4377014068 hasRelatedWork W2518028869 @default.
• W4377014068 hasRelatedWork W2594816880 @default.
• W4377014068 hasRelatedWork W2973632916 @default.
• W4377014068 hasRelatedWork W3003914988 @default.
• W4377014068 hasRelatedWork W3011472052 @default.
• W4377014068 isParatext "false" @default.
• W4377014068 isRetracted "false" @default.
• W4377014068 workType "article" @default.